肺癌组织中端粒酶活性研究

Study of the Telomera se Activity in Human Lung Cancer

摘要: 目的　探讨端粒酶作为原发性肺癌肿瘤标记物的可能性。方法　采用 TRAP法检测了 81例纤维支气管镜活检组织端粒酶活性, 包括 50例肺癌组织, 2 4例慢性粘膜炎组织, 7例化生、不典型增生组织。结果　 50例原发性肺癌组织中, 有 41例端粒酶活性表达阳性, 其阳性率为 82 % ;7例不典型增生组织中端粒酶活性表达阳性 4例, 2 4例慢性粘膜炎标本中, 2例端粒酶表达阳性。结论　端粒酶是一种特异性的恶性肿瘤基因标志物。

Abstract: Objective To investigate the possibility of using telomerase as a tumor marker for human primary lung cancer. Methods 81 human lung specimens, comprising 50 carcinoma tissues, 24 chronicmucit is tissues and 7m etaplasia or atypia tissues,were examined for telomerase activity by TRAP assay. Results 41 of 50 the carcinoma tissue specimens were positive for telomerase activity with a positive rate of 82%, 4 of 7 atypical tissue specimens and two of 24 chronic mucitis tissue specimens were positive. Conclusion Telom erase is a tumor specific gene marker.