Abstract: Objective 　To study the effect s of zoledronic acid on adhesion, migration, invasion and expres2 sion of mat rix metalloproteinase (MMP22, MMP29) of osteosarcoma LM8 cell line. Methods 　Af ter LM8 cells were t reated with zoledronic acid, Cell Counting Kit28 assay was used to assess LM8 cells ad2 hesion and the effect of zoledronic acid on viability of LM8 cells ; their migration and invasion to ECM were measured by wound assay and t ranswell chamber method respectively. The content s of MMP22 and MMP29 in culture supernatant were detected with EL ISA. Results 　At the concent ration of 6. 25μmol/ L, 12. 5μmol/ L, 25μmol/ L, zoledronic acid significantly inhibited LM8 cells adhesion, migra2 tion and invasion to the ECM. In addition, the effect of zoledronic acid on viability of LM8 cells was neg2 ligible. Zoledronic acid significantly inhibited the expression of mat rix metalloproteinase (MMP22, MMP2 9) . Conclusion 　Zoledronic acid has anti2adhesion, anti2migration and anti2invasion effect s on osteosarco2 ma LM8 cells in vi t ro. In addition, zoledronic acid can result in the down2regulation of expression of ma2 t rix metalloproteinase (MMP22, MMP29) .