p53 基因突变及血管内皮生长因子表达与大肠癌肝转移的关系
Factor Expression with Hepatic Metastasis of Large Intestinal Carcinoma
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摘要: 目的 探讨 p53基因突变及血管内皮生长因子 (VEGF)表达与大肠癌肝转移的关系。方法 采用 PCR- SSCP及 DNA测序方法检测大肠癌原发灶、癌旁肠粘膜及肝转移灶组织 p53基因突变。用 RT- PCR方法检测 VEGF表达。结果 64例大肠癌原发灶、癌旁肠粘膜、肝转移灶中p53基因突变率分别为 65.6%、9.1 %、87.1 %。肝转移组与无肝转移组原发灶的 p53突变率分别为87.1 %、45.5%, 肝转移组原发灶中 p53突变率较高 (P<0 .0 5)。在癌原发灶、癌旁肠粘膜、肝转移灶中 VEGF表达阳性率分别为 46.9%、7.8%、1 0 0 %。肝转移灶中 VEGF表达阳性率高于其它组织 (P<0 .0 5)。在肝转移组原发灶和肝转移灶中 p53突变和 VEGF表达均为阳性的检出率也较高。结论 p53基因突变和 VEGF增强表达与大肠癌肝转移的发生有密切关系。Abstract: Objective To study relation of mutant p53 and vasvular endothelial growth factor(VEGF) expression with hepatic metastasis f large intestinal carcinoma. Methods PCR-SSCP and DNA seqencing techniques were used to detect p53 mutation and VEGF was examined with RT-PCR in primary foci,paratumoral tissues and hepatic metastasis of 64 cases of colorectal cancer. Results In 64 cases the mutant rates of p53 in primary foci、paratumoral tissues and hepatic metastasis were 65.6%,9.1%,87.1% respectively.In Hepatic metas...
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