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用BC2单抗检测胃癌及癌前病变组织中MUC1基因表达及其意义

Expression of MUC1 Gene in the Tissues of Gastric Carcinoma and Pre-cancerous Lesion with BC2 Antibody and its Significance

  • 摘要: 目的 揭示胃癌及肠化组织中MUC1基因的表达与其临床病理行为之间的联系。方法 用BC2抗体和免疫组化SP法检测人正常胃肠道粘膜、肠化粘膜以及胃癌组织中MUC1基因核心肽的表达。结果 人正常胃粘膜组织中广泛分布MUC1基因产物(100%),抗原主要位于上皮层和胃腺的中下部;肠化和胃癌组织中的表达阳性率分别为79.3%和82.6%;胃癌组织中MUC1基因表达与患者的性别、部位、大小、淋巴结转移、分化类型、浸润深度、临床分期和Lauren氏分型之间无关(P>0.05);但MUC1基因强阳性者与中弱阳性者之间的临床分期存在明显的差别(P<0.05),表达越强,Ⅰ~Ⅱ期的可能性越小;不同类型肠化中MUC1基因的表达无差异(P>0.05)。结论 上述结果提示用BC2抗体检测的MUC1基因可能是胃癌进展的有用标志,可能与胃癌患者的临床病理行为之间有关,而与肠化分型无关。

     

    Abstract: Objective To elucidate the association between expression of MUC1 gene in the tissues of gastric carcinoma and intestinal metaplastia and its clinicopathological factors. Methods MUC1 core protein was detected in the normal gastrointestinal mucosa and tissues of intestinal metaplasia and gastric carcinoma with BC2 antibody by immunohistochemical SP method.Reaults MUC1 core protein was diffusely distributed in human normal gastric mucosa(100%),mainly located in the perinuclear area of epithelial cells and cytoplasm of mucous neck cells and basal glandulae,the positive rates of core protein in the tissues of intestinal metaplasia and gastric carcinoma were 79.3%,82.6% respectively.No association was found between the expression of MUC1 gene in the cancerous tissues and the patients'sexes,locations and size of cancerous tissues,metastases of lymph node,types of differentiation,depth of invasiveness,clinical stages and lauren's types(P>0.05).But,the number of clinical stages Ⅰ~Ⅱ was significantly lower in those patients whose cancerous tissues were strongly positive staining than those with moderately and weakly posivive staining (P<0.05),No difference was found between expression of MUC1 gene and different types of intestinal metaplasia (P>0.05). Conclusion These results indicated that MUC1 core protein detected by BC2 antibody might be a useful marker for the progression of gastric carcinoma and associated with its clinicopathological factors,but no association with types of intestinal metaplasia.

     

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