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8例胃类癌光镜、电镜、组织化学及DNA含量的观察

Observation of Histopathology,Ultrastructure,Histochemistry and DNA Ploidy of Eight Cases of Gastric Carcinoid Tumor

  • 摘要: 目的 探讨胃类癌的组织学、组织化学、超微结构特点和DNA含量的变化。方法 对8例胃类癌组织学标本作光镜、电镜观察,AB-PAS、Masson Fontana、Grimelius染色和DNA含量的测定。结果 光镜下胃类癌有其明显的组织特征,AB-PAS染色显示胃粘膜有中、重度肠上皮化生,Masson Fontana染色全部为阴性,Grimelius染色6例类癌细胞浆有棕色或黑色阳性颗粒,电镜观察:类癌细胞胞浆内有大小不一、群集和散布高密度电子颗粒,DNA含量测定:类癌细胞主要为二倍体和四倍体细胞。结论 “典型”类癌通过组织学观察即可确诊,“非典型”类癌需结合电镜检查确诊。

     

    Abstract: Objective To explore the characteristics of histopathology,ultrastructure and histochemistry and changes of DNA ploidy of gastric carcinoid tumors. Methods Eight cases of gastric carcinoid tumors were studied by microscope,electron microscope,AB-PAS stain,Masson Fontana stain,and Grimelius stain,and also by image analysis system for DNA ploidy. Results Microscope observation showed characteristic histopathological change,AB-PAS stain showed moderate to severe intestinal metaplasia of stomach mucosa.All of cases showed negative of Masson Fantana stain,and six cases showed positive argyrophilic staining granules of Grimelius stain in carcinoid cytoplasm.Ultrastructurally,there were numerous dense-core pleomorphic secretory granules scattered throughout the cytoplasm.A large number of carcinoid cells were diploid and tetraphoid by means of DNA ploidy analysis. Conclusion “Classic” gastric carcinoid tumor was diagnosed only by histopathologic observation,but diagnosis of“non-classic” gastric carcinoid tumor also need to use other pathologic techniques such as electron microscope,etc.

     

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