Abstract: Objective 　To predict the HLA-A2/A3-rest ricted CTL epitopes of COX-2 and MAGE-4 hyperexpressed in esophageal carcinoma ( ECC) . Methods 　The HLA-A *0201/A3 rest ricted CTL epitopes of objective antigens were predicted by SYFPEITHI prediction method combined with the MHCPred and NetChop 3. 0. All peptides were compared with reported CTL epitopes. Results 　The SYFPEITHI prediction values of all possible nonamers of the two proteins sequence were added together and the over 20-score peptides were chosen for further analysis in primary prediction. 42 candidates of CTL epitopes (nonamers) derived from the primary prediction results were selected by analyzing with the MHCPred and NetChop3. 0 methods. Conclusion 　All these peptides have not been reported. The 42 candidates of CTL epitopes will benefit the identification of CTL epitopes by experiment . These nonamers may be useful in the design of therapeutic peptide vaccine for ECC and as immunotherapeutic st rategies against ECC after identified by immunology experiment . The peptides MAGE-4 _22-30、_202-210、_286-294 and COX-2 _479-487 have the potential cross-immunity with HLA-A2 and HLA-A3 supertype, which will provide candidates for efficient and broad-spectrum vaccine.