应用FISH 和组织芯片方法研究肺癌组织Ets1 mRNA的表达及意义
Expression and Significance of Ets1 mRNA in Lung Cancer Tissue Microarray ( TMA) Detected by FISH Method
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摘要: 目的 用荧光原位杂交（fluorescence in situ hybridization, FISH）方法检测肺癌组织芯片（tissue microarray, TMA）中Ets1 mRNA表达情况,并探讨其在肺癌发生、发展中的作用和意义. 方法 利用mRNA FISH方法和组织芯片技术,检测肺癌Ets1 mRNA的表达情况. 结果 肺癌组中有70.4%表达Ets1 mRNA,而正常肺标本中未见Ets1 mRNA表达,差异有统计学意义（P〈0.05）;Ets1 mRNA的表达与肺癌的分化程度、淋巴结转移和临床分期显著相关（P〈0.05）. 结论 组织芯片技术可以使实验简便、经济和标准化;FISH方法敏感性强,稳定性高,荧光染色保存时间长,可以较好地反映mRNA在细胞中的定位和含量.本研究发现Ets1 mRNA表达与肺癌的侵袭转移有关,因而Ets1基因可能成为肿瘤患者预后判断和指导临床治疗的重要指标.Abstract: Objective To observe the expression of Ets1 mRNA in lung cancer tissue microarray by FISH method, and investigate the role and significance of it in lung cancer genesis and progress. Methods The expression of Ets1 mRNA in lung cancer was detected by tissue microarray technology and fluorescence in situ hybridization ( FISH) method. Results 70. 4 % of lung cancer samples expressed Ets1 mRNA ; the positive rate of lung cancer samples was significantly higher than that of normal lung tissue ( P <0. 05) ; the expression of Ets1 mRNA was related to the differentiation degrees, lymph node metastasis and clinical stages ( P < 0. 05) . Conclusion Tissue microarray technology has many advantages, such as high efficiency, convenience, economy and standardization ; FISH is sensitive, steady, and preservative. Ets1 mRNA was highly expressed in lung cancer ; it was related to the progress and malignant behavior ; it may play an important role in lung cancer genesis and progress and offer basis to prognosis estimate and t reatment .
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