鲨鱼软骨制剂抑癌作用与诱发胃癌细胞凋亡
The Antitumor Activity of Shark Cartilage Preparationis Associated with Apoptosis
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摘要: 目的 研究鲨鱼软骨制剂 (SCP)对人胃癌细胞 (MGC80 - 3)生物学行为的影响并探讨其抗癌作用机制。方法 用不同浓度的 SCP加入体外培养的 MGC80 - 3细胞系中, 用 MTT比色法, 荧光显微镜, 透射电子显微镜技术和琼脂糖凝胶电泳方法, 观察 MGC80 - 3细胞形态学和生化方面的改变。结果 SCP明显抑制 MGC80 - 3细胞生长, IC50 值为 1 mg/ ml;荧光显微镜下可见MGC80 - 3细胞在形态学上出现典型的核固缩、破裂等凋亡细胞的形态学改变 ;透射电镜下可见细胞核染色质凝集呈帽状分布于核膜下, 凋亡细胞附近可见多个高电子密度的凋亡小体 ;琼脂糖凝胶电泳呈现典型的“梯状”(DNA ladder)带。结论 SCP诱导人胃癌细胞凋亡是其抑制肿瘤作用机制的重要方面之一。Abstract: Objective To study the biological behavior changes and its mechanism of shark cartilage preparation(SCP) on human gastric cancer cells(MGC80 3). Methods Using experiment in vitro,we added SCP of different concentrations into the medium in which the cells were cultured.The apoptosis was detected by fluorescent light microscope,transmission electron microscope and gel electrophoresis. Results The effect of SCP on the growth of MGC80 3 cells was evaluated by MTT assay.The IC\- 50 Values of SCP for MGC80 3 c...
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