HIF-1α和Glut-1在乳腺癌中的表达及其与血管生成的关系
Relationship of Expression of HIF-1α and Glut-1 with Angiogenesis in Breast Carcinoma
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摘要: 目的探讨HIF-1α、Glut-1在乳腺癌中的表达及其与微血管密度的关系。方法免疫组化法检测20例癌旁组织、60例乳腺癌中HIF-1α、Glut-1表达,用CD34抗体标记乳腺癌血管内皮细胞,计算微血管密度(MVD)。结果HIF-1α、Glut-1在乳腺癌中阳性表达分别为68.3%、63.3%,均高于对照组,差异有统计学意义(P<0.01),两者表达呈正相关(r=0.461,P<0.001)。HIF-1α、Glut-1阳性的乳腺癌组织MVD高于阴性者,差异有统计学意义(P<0.01),HIF-1α、Glut-1均阳性时MVD最大(P<0.01),HIF-1α、Glut-1、MVD与肿瘤临床分期、淋巴结转移密切相关(P<0.01)。结论乳腺癌组织中HIF-1α、Glut-1呈过表达,两者在其发生、发展过程中可能具有协同作用,对肿瘤新生血管生成可能有重要作用。Abstract: Objective To study the relationship of the expression of HIF-1αand Glut-1 with Angiogenesis in breast carcinoma,and to analyze the correlation of them. Methods HIF-1α and Glut-1 protein expression were detected by immunohistochemical methods in 60 cases of breast carcinoma and 20 cases of tumor adjacent normal breast tissue.CD34 marking vascular endothelialcell was used to measure the microvascular density(MVD). Results The positive expression rates of HIF-1α and Glut1 in breast carcinoma was 68.3% and 63.3%,showing a significantly difference in the expression of HIF-1αand Glut-1 between breast carcinoma and cont rol group ( P < 0. 01), Expression of HIF-1αwas positively correlated with expression of Glut-1 ( r = 0. 461, P < 0. 001) . MVD in HIF-1α( + ) or Glut-1 ( + ) group was significantly higher than that in HIF-1α( - ) or Glut-1 ( - ) group ( P < 0. 01) . The MVD reached the maximum in patient s whose HIF-1αand Glut-1 were both positive ( P < 0. 01) . The expression of HIF-1α, Glut-1 and MVD were significantly correlated with lymphatic metastasis and surgical-pathologic staging ( P <0. 01) . Conclusion There have overexpresion of HIF-1αand Glut-1 in breast carcinoma, they may have a synergistic effect in the genesis and development of breast carcinoma, and may play an important role in angiogenesis of breast carcinoma.
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