不同胃粘膜病变中端粒酶活性与幽门螺杆菌感染的关系
The relationship between reactivity of telomerase and helicobacter pylori infection in different lesions of gastric mucosae
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摘要: 目的 研究端粒酶催化亚单位 (hTERT)的活性与幽门螺杆菌 (Helicobacterpylori,Hp)感染的关系。方法 应用组织化学、免疫组化和原位杂交方法, 对 4 2例慢性胃炎 (CG)、81例肠上皮化生 (IM )、75例胃上皮不典型增生 (GED)及 84例胃癌 (GC)组织中的Hp感染及hTERT活性进行检测。结果 (1)从CG→IM→GED→GC, 其hTERTmRNA的表达从无到有, 由低到高。GC中表达率最高, 分别显著高于IMⅢ和GEDⅡ～Ⅲ (P <0 .0 1)。 (2 )在GEDⅡ～Ⅲ和肠型GC组, Hp阳性病例中hTERTmRNA表达率均分别明显或显著高于Hp阴性病例 (P <0 .0 5 ;P <0 .0 1)。结论 在不同胃粘膜病变中, 主要是在胃上皮中～重度不典型增生及肠型胃癌中, Hp感染与hTERT活性密切相关。Hp感染可通过激活端粒酶而使细胞永生化的途径导致胃癌的发生。Abstract: Objective To investigate the relationship between reactivity of catalytic protein submit of human telomerase reverse transcriptase (hTHER) and helicobacter pylori(Hp) infection. Methods Histochemical staining,immunohistochemistry and in situ hybridization were conducted to detect Hp infection and reactivity of hTERT in 42 chronic gastritis (CG), 81 intestinal metaplasia (IM), 75 gaseric epithelial dysplasia (GED) and 84 gastric carcinoma (GC). Results (1)The expression of hTERTmRNA increased with the devel...
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