银杏叶提取物对胶质瘤细胞NF-κB表达和NO生成的调控

Regulatory Effect of Ginkgo Biboba Extract on the Expression of NF-κB and Nitroxide Production in Glioma Cells

摘要: 目的研究银杏叶提取物(EGb761)对C6胶质瘤细胞核转录因子-κB(NF-κB)表达和可诱导型一氧化氮合酶(iNOS)、一氧化氮(NO)生成的影响。方法以脂多糖(LPS)和佛波酯(PMA)诱导体外培养的C6胶质瘤细胞表达可诱导型一氧化氮合酶、产生大量NO,应用激光共聚焦成像系统和NO荧光探针监测细胞内NO浓度变化,逆转录基因扩增技术和蛋白质印迹技术检测EGb761对C6胶质瘤细胞中iNOS基因表达的影响,并探讨这一调控作用的分子机制。结果EGb761能明显降低C6胶质瘤细胞中iNOSmRNA和蛋白的表达、减少NO的生成,抑制IκB-α的降解和阻止p65/RelA进入细胞核。结论EGb761可通过NF-κB信号通路对C6胶质瘤细胞iNOS基因表达和NO的生成进行调控。

Abstract: Objective To study the effects of Ginkgo biloba extract(EGb761) on the expression of nuclear factor-kappa B(NF-κB), contents of nitric oxide(NO) and inducible nitric oxide synthase(iNOS) in C6 glioma cells. Methods Upon stimulation with lipopolysaccharide(LPS) and phorbol-1,2-myristate-1,3-acetate (PMA), iNOS gene was expressed in cultured C6 glioma cells, which caused the consequent generation of high concentration of nitric oxide. The effects of EGb761 on the intracellular NO concentration and the express...