~(60)Co照射对肿瘤坏死因子基因转导的人肝癌细胞的增殖及TNF-α分泌的影响
The effect of ~(60)Co irradiation on the proliferation of and TNF-α secreting in TNF-α gene-transduced human liver cancer cells
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摘要: 目的 用60 钴 (60 Co)照射肿瘤坏死因子基因转导的人肝癌细胞 (BEL 74 0 4 TNF细胞 ), 用于制备一种肿瘤疫苗。方法 用不同方式及不同剂量的60 Co照射BEL 74 0 TNF细胞及未转基因的BEL 74 0 4细胞, 观察照射后细胞增殖情况及肿瘤坏死因子α(TNF α)分泌量。结果 经 80Gy 60 Co连续分量照射的BEL 74 0 TNF细胞增殖能力丧失, 但仍然生存并能持续分泌TNF α2周以上, 细胞在 3周内全部死亡。结论 用60Co照射BEL 74 0 4 TNF细胞制备肿瘤疫苗是可行的, 80Gy 60Co连续分量照射可能是合适剂量Abstract: Objective To make a tumor vaccine using 60 Co irradiated human tumor necrosis factor alpha (TNF α) gene transduced human liver cancer cells(BEL 7404 TNF cells). Methods 60 Co were applied in different ways and different dosages to irradiate BEL 7404 TNF cells and the parented BEL 7404 cells, and the growth and the secreting amount of TNF α of tumor cells were observed after irradiating. Results The BEL 7404 TNF cells were alive and preserved the ability to secrete TNF α f...
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