BRM释介素抗肿瘤作用研究

Antitumor effect research of BRM

摘要: 目的　研究中药复方胶囊BRM释介素的抗癌活性及诱导人神经胶质瘤细胞SHG 4 4凋亡。方法　运用MTT法检测BRM对体外人癌细胞株的抑制作用 ;运用小鼠移植瘤模型观察BRM体内抑瘤活性 ;应用电镜、流式细胞仪分析、琼脂糖凝胶电泳观察BRM诱导SHG 4 4。结果　BRM释介素体外对SHG 4 4、MCF 7具有明显的抑制作用 ;体内对小鼠移植性肿瘤表现出较显著的抗癌活性 ;SHG 4 4在BRM水提液作用下发生细胞凋亡。结论　BRM释介素的抗肿瘤活性与诱导细胞凋亡有关

Abstract: Objective To evaluate the antitumor action of BRM, and its mechanism. Methods Inhibition tumor experiment study on mice which was transplant tumor cell strains in mice subcutaneous tissue in vivo and MTT method tested human tumor cell lines in vitro and tumor cells include cell electric morphology, flow cytometry, agarose gel electrophoresis, were performed for determining apoptosis in tumor cells. Results It was found that BRM had a significant inhibition rate of tumor reproduce in vivo and in vitro. Mea...