Abstract: Objective 　To explore the different cont ribution of resveratrol (Res) to the proliferation and apoptosis of glioma U251, U87 and SHG-44 cells in vitro, verify the apoptosis of U251 cell caused by Res. Methods 　MTT assay was used to measure it' s effect s on the proliferation of U251, U87 and SHG-44 cells cultured with different concent rations of Res for 24h and it' s effect s on the proliferation of U251, U87 and SHG-44 cells for 6h, 24h and 48h. The Annexin VFITC and PI assay by flow cytometer were applied to detect the apoptosis of U251, U87 and SHG-44 cells. HE staining, Hoechst 33342 fluorescence staining, transmission elect ron microscope ( TEM) was used to observe the changes of U251 cellular morphous. The cell cycle change of U251 cells was detected by FCM. Results 　Resobviously suppressed the proliferation of U251, U87 and SHG-44 giloma cells ( P < 0. 01), and the suppressive effect concent ration-dependent manner, and the apoptosis rate of U87 cell was higher than that of U251 and SHG-44 cells. Rescaused U251 cell cycle mainly with G arrest . Conclusion 　Res caused U87 cells more significant cytotoxicity and apoptosis than U251 and SHG244 cells, Res induced U251 cell cycle mainly with G arrest .