多西他赛联合放疗对大鼠脊髓损伤的观察
Pathology of Radiation Injury to the Rats Spinal Cord Induced by Combined Treatment with Docetaxel and Radiation
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摘要: 目的探讨多西他赛联合放射治疗对大鼠脊髓损伤的影响。方法将40只Wistar品系大鼠随机分为5个组,即空白组、多西他赛组、放疗组、先多西他赛后放疗组和先放疗后多西他赛组。多西他赛组单次腹腔注射多西他赛5mg/kg。放疗组、先多西他赛后放疗组和先放疗后多西他赛组采用Varian2300C/D直线加速器6MeV电子线照射大鼠胸段脊髓,照射野为2cm×4cm,5Gy/次,隔日1次,总剂量为40Gy。联合组多西他赛与照射的间隔时间为3d。照射结束后5个月,行活体灌注固定,取材,光镜和电镜观察脊髓的形态学变化。结果光镜下,放疗组和先多西他赛后放疗组白质部分坏死;先放疗后多西他赛组白质大片坏死。后2组均见胶质细胞核固缩。各组灰质内神经元和血管未见变化。电镜下,各组脊髓髓鞘改变程度不一。放疗组脊髓髓鞘板层结构水肿、粘连,模糊不清。先多西他赛后放疗组的脊髓髓鞘的板层结构部分松解、扭曲;胶质细胞核固缩。先放疗后多西他赛组脊髓损伤髓鞘的板层结构大部分松解、扭曲、断裂;细胞器严重变形;胶质细胞凋亡。各组血管均未见明显改变。结论多西他赛与放疗联用明显加重脊髓损伤,且放疗后加用多西他赛脊髓损伤加重。Abstract: Objective To explore the injury of normal spinal cord irradiated by the combination of docetaxel and radiotherapy. Methods In this experiment, 40 Wistar rats were divided groups as following: normal, docetaxel, radiation, radiation after docetaxel, docetaxel after radiation. Docetaxel was administrated singlely 5mg/kg. The thoraxs of rats were irradiated with 6MeV electron beam of Varian 2300 C/D line accelerater. The dose of irradiation was 5Gy singlely, and total dose was 40Gy. The size of field was 2cm×4...
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