恶性胸水中IL-2水平与TIL细胞体外扩增及活性的关系
The influence of the level of IL-2 in pleural fluid on the increasing rate and anti-tumor effect of TIL derived from malignant pleural fluid in vitro ReplaceChar('spanTitle')
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摘要: 目的 通过对恶性胸水中内源性白介素 2的水平进行检测及对胸水中肿瘤浸润淋巴细胞体外增殖能力及体外抗肿瘤活性的观察, 了解内源性IL 2水平对胸水TIL细胞体外扩增及抗肿瘤活性的影响。方法 用MTT法检测TIL细胞体外杀瘤活性, 用ELISA法检测IL 2的水平。将IL 2水平较高的2 4例患者分为一组, IL 2水平较低的 2 4例患者分为另一组。比较两组患者胸水TIL细胞体外增殖速度及杀瘤活性。结果 恶性胸水中内源性IL 2水平较低组, TIL细胞体外扩增倍数明显高于内源性IL 2水平较高组。IL 2水平较低组TIL细胞体外杀瘤率明显高于IL 2水平较高组。结论 恶性胸水中内源性IL 2水平高低与TIL细胞体外增殖的速度及其杀瘤活性明显相关, 原有IL 2水平较低的患者胸水中TIL细胞体外增殖的速度较快, 杀瘤活性较强。胸水中IL 2水平可望作为恶性胸水IL 2 /TIL治疗选择的指标。Abstract: Objective To explore the influence of the level of IL-2 in pleural fluid on the increasing rate and anti-tumor effect of tumor-infiltrating lymphocytes (TIL) derived from malignant pleural fluid in vitro. Methods The levels of IL-2 in pleural fluid were measured by ELISA. MTT assay was used to detect the cytotoxicities against Lovo cell line of the TILs. Results The increase rate of TIL at the group with lower level of IL-2 was rapid than that of group with higher IL-2 level. The cytotoxicities against Lovo cell lines at the group with lower level of IL-2 were stronger than that of group with higher IL-2 level. Conclusion The level of IL-2 in pleural fluid relates to the increasing rate and cytotoxicity against tumor cells of the TILs. ReplaceFont
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