微缺氧对三氧化二砷抑制胃癌细胞SGC-7901生长的影响

张俊文; 王丕龙

doi:10.3971/j.issn.1000-8578.2237

微缺氧对三氧化二砷抑制胃癌细胞SGC-7901生长的影响

Inhibitoy Effect of Arsenic Trioxide on Gastric Cancer Cell SGC-7901 During Hypoxia

摘要

摘要: 目的常氧和微缺氧条件下三氧化二砷（As₂O₃）注射液对人胃癌细胞株SGC-7901生长抑制作用的对比研究。方法运用产气袋法（GasPaK）制造微缺氧条件，实验分为微缺氧组和对照组，用MTT法检测药物效应。流式细胞仪分析细胞周期和细胞凋亡。结果不同浓度As₂O₃对人胃癌细胞株SGC-7901生长有抑制作用，并呈剂量一效应关系。细胞滞留于G2／M及S期。微缺氧条件下As₂O₃对人胃癌细胞株SGC-7901生长抑制作用下降（P〈0．01）。凋亡细胞百分比下降。结论 As₂O₃对人胃癌细胞生长有抑制作用，但对缺氧胃癌细胞的抑制作用下降，单用As₂O₃治疗胃癌存在一定的局限性。

Abstract: Objective 　To investigate the effect of arsenic t rioxide (As₂O₃ ) on gast ric cancer cell SGC-7901 during the normal and hypoxia. Methods 　The environment of hypoxia was established by GasPak method. The effect of As₂O₃ was determined by MTT, and FCM was used for studying the apoptosis of SGC-7901 and cell cycle. Results 　As₂O₃ can inhibit significantly the growth of SGC-7901, and arrest the cell in G2/M and S phases. But this inhibitory effect was decreased significantly during hypoxia ( P < 0. 01), which was found by the lower percentage of apoptosis. Conclusion 　As₂O₃ can inhibit the growth of gastric cancer cell, but during hypoxia this inhibitory effect was decreased, so it may be limited to cure hypostatic neoplasm for only using As₂O₃ .

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