Abstract: Objective 　To study the mechanism and effect of gensenoside Rg3 on Hep2 Cell Line during the normoxia and hypoxia. Methods 　Anoxic cultured Hep2 human laryngeal cancer cell line, mean-while the normal cont rol group and positive cont rol group (DDP) were set . The cell apoptotic was detected by TUNEL method. The cell cycle and cell apoptosis analysis was detected by FCM. Then the expression of HIF-1αand VEGF protein was detected by immunohistochemistry and flow cytomet ry ; the expression of HIF-1αand VEGF mRNA was detected by t ranscription-polymerase chain reaction (RT-PCR) . Results 　The apoptosis rate obviously increase by TUNEL and FCM( P < 0. 05) . Compared with the Hep2 cells with-out any induction, the expression of HIF21αand VEGF protein in Rg3 group s and DDP groups obviously depressed ( P < 0. 05) ;the expression of HIF21αand VEGF mRNA in Rg3 group s obviously depressed ( P < 0. 05) ;but had no effect on the level of HIF21α and VEGF mRNA in DDP groups. Conclusion Rg3 could inhibit the high protein and mRNA expression of HIF-1αand VEGF and it could induce cell apopto-sis under anoxic conditions, which may be one of it s anticancer mechanisms.