南蛇藤素对肿瘤血管的抑制作用

Experimental Study on Inhibiting Angiogenesis in Tumor by Celastrol

摘要: 目的探讨南蛇藤素对肿瘤血管抑制作用。方法通过建立动物肿瘤模型，对南蛇藤素予以实验治疗，结合免疫组化，检测其抑瘤率和肿瘤内血管密度，以及瘤体VEGF和bFGF的表达的改变。结果南蛇藤素具有明显的抑制S180肉瘤作用，其3rag／kg剂量组抑瘤率达46．86％，与5-Fu联用抑瘤率达60．2％，联合给药组和南蛇藤素3mg／kg剂量组的微血管密度、VEGF、bFGF的表达均明显低于对照组间和5-Fu组，差异有显著性（P〈0．01）。结论南蛇藤素对小鼠S180肉瘤血管有一定的抑制作用，降低VEGF、bFGF的表达可能是其抑制肿瘤血管形成的机制之一。

Abstract: Objective 　To investigate the effect of inhibiting angiogenesisby celastrol. Methods 　The tumor inhibitory rate and the vascular density were tested in animal tumor model with experimental t reatment . The expression of VEGF and bFGF were measured by immunohistochemist ry. Results 　The tumor inhibitory rate was 46. 86 % in celast rol (3mg/ kg), and was 60. 2 % in combined group . The int ratumoral microvessel density(MVD), expression of VEGF and bFGF in combined group and celastrol (3mg/ kg) group were lower than the cont rol and 5-Fu group ( P < 0. 01) . Conclusion 　Celast rol have inhibitory effect on S₁₈₀sarcoma and strong inhibitory effects on angiogenesis. Its mechanism may be associated with the down-regulating of VEGF and bFGF expression.