人参皂甙Rg3 抑制肿瘤新生血管形成机制研究

Inhibitory Effect of Tumor Induced Angiogenesis by Ginsenoside Rg3

摘要: 目的　人参皂甙 Rg3抑制肿瘤新生血管的机制。方法　采用鸡胚 CAM和小鼠 Lewis肺癌模型及免疫组化方法探讨 Rg3抑制新生血管形成的机制。结果　Rg3浓度为 0 .1 m M、0 .5 m M时, 抑制鸡胚 CAM的血管指数 (BI)分别达到 6 2 .4%、45 .6 %。而给予小鼠 Rg3灌胃剂量分别为 5、1 0、2 0 mg/kg/隔日, 连续 2 0天后, 观察到 3组的抑瘤率分别为 2 3.0 4%、31 .30 %、47.1 0 %, 同时观察到 Rg3组的新生血管数也明显减少。免疫组化证实 Rg3可明显抑制肿瘤组织内 b FGF的表达。结论　Rg3可明显抑制 Lewis肺癌的生长并抑制了肿瘤新生血管形成。

Abstract: Objective The aim of the present study was to examine the mechanism of Inhibitory effect of ginsenoside Rg3 on tumor growth. Methods We investigated the inhibitory effect of Rg3 on tumor neovascular by the method of immunohistochemistry,chick chorioallantoic membrane(CAM) and Lewis lung carcinoma model.,Results We demonstrate that Rg3 can obviously inhibits angiogenesis of the CAM with a Bl=62,4~45,6.Treatment with Rg3 in vivo can obviously inhibits Lewis lung carcinoma growth with the inhibition ratio from ...