血清神经节苷脂快速测定法及其初步应用

A fast method to determine serum gangliosides and its primary application

摘要: 目的　建立GLS快速测定法并研究其临床价值。方法　从血清提取糖脂, 间苯二酚 -HCl与GLS特异糖基反应, 测定吸光度。结果　健康人血清GLS含量 5 81± 90mg/L(40 0～ 76 0mg/L)。头颈恶性肿瘤患者GLS(90 8± 2 2 6mg/L)显著增高 ;良性肿瘤和非肿瘤患者GLS(6 0 2± 12 8与 6 95± 16 1mg/L)则不增高。该法对头颈恶性肿瘤敏感度、特异度、准确度、诊断效率为 73.8%、88.6 %、81.4 %、6 5 .4 %。结论　GLS快速测定法简便、稳定、可靠 ;测定GLS对头颈恶性肿瘤诊断具有显著临床价值。

Abstract: Objective To establish a fast method to determine gangliosides (GLS) and its clinical value. Methods The serum glycolipids were extracted and the specific glycoside on gangliosides was reacted with resorcin-HCl and assayed with spectrophotometer. Results The serum GLS of healthy people was (581±90)mg/L. It was significantly increased in head and neck malignant tumors patients (908±226mg/L), and not evidently increased in benign tumors (602±128mg/L) and other diseases (695±161mg/L) patients. To diagnose h...