鼻NK/T细胞淋巴瘤的免疫表型、EBV感染及TCRγ基因重排的检测
Immunophenotype and Epstein-Barr Virus Infection and Detection Rearrangement of T Cell Receptor γ(TCRγ) Gene in Nasal NK/T-cell Lymphoma
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摘要: 目的 检测鼻NK/T细胞淋巴瘤（NK/TCL）的免疫表型、EBV感染及TCRγ基因重排,为诊断和鉴别诊断提供依据。方法 收集诊断鼻NK/TCL48例患者石蜡包埋标本,用免疫组化SP法标记LCA、CD79α、CD20、CD56、CD3、CD45RO及EBV抗体研究其免疫表型;EBER探针原位杂交方法检测EBV编码的小分子RNA（EBER）;聚合酶链式反应扩增方法检测TCRγ基因重排。结果 48例鼻NK/TCL均表达LCA,CD3、CD45RO、CD56和EBV阳性率分别为44%、52%、73%和19%,CD79α和CD20均阴性;EBER阳性率为81%;TCRγ基因重排阳性率为19%。结论 鼻NK/TCL免疫表型不一致,并非所有病例CD56阳性,石蜡切片中CD3阳性定位于细胞质;EBER在肿瘤细胞中高表达,提示它们可能为NK细胞来源;部分TCRγ基因重排阳性病例应为鼻NK样T细胞淋巴瘤。Abstract: Objective To explore the immunophenotype and Epstein-Barr Virus(EBV) infection of nasal NK/T cell lymphoma and the significance of detection rearrangement of TCR γ gene in diagnosis and differential diagnosis of nasal NK/T cell lymphoma. Methods Fouty-eight cases of nasal NK/T cell lymphoma were studied, immunophenotype was analyzed by immunohistochemical staining for LCA、CD79α、CD20、CD56、CD3、CD45RO and EBV with SP method. In situ hybridization (ISH) with EBER1/2RNA probes was perfomed.TCR γ gene rearrangeme...
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