Abstract: Abstract: Objective To investigate the expression of transcription factor forkhead box protein O (FOXOs) gene in gastric cancer tissues and its correlation and clinical significance with Helicobacter pylori (Hp) infection. Methods The expression of FOXOs (including FOXO1, FOXO3, FOXO4 and FOXO6) genes was detected by immunohistochemistry in the cancerous tissues and paracancerous tissues of 41 patients with Hp (-) and 29 patients with Hp (+) gastric cancer, as well as in the gastric tissues of 30 normal subjects, and the correlation between the gene expressions and the Hp infections and clinicopathological features was investigated by Kaplan-Meier The Kaplan-Meier plotter database was used to analyze the relationship between the expression of FOXO3, FOXO4 and FOXO6 genes and the survival and prognosis of gastric cancer patients. Results Compared with Hp(-) gastric cancer tissues, the expression levels of FOXO1, FOXO4 and FOXO6 were high in Hp(+) gastric cancer tissues (P<0.05); whereas the expression levels of FOXO1, FOXO3 and FOXO4 in cancer tissues of gastric cancer patients with Hp(+) were lower than that in paracancerous tissues (P<0.05) and normal tissues (P<0.0001).The expression rates of FOXOs in gastric cancer tissues were not correlated with patients' gender, age and tumor size (P> 0.05), but closely correlated with the degree of differentiation, depth of infiltration, lymph node metastasis and TNM stage of gastric cancer (P< 0.05). Meanwhile, FOXO1/3 was associated with survival prognosis of gastric cancer patients. Conclusion Hp infection promotes the expression of FOXO1/4/6 in gastric cancer tissues, and the high expression of oncogenes FOXO1/4 may be one of the reasons why gastric cancer patients with Hp(+) usually have a better prognosis; the FOXOs genes are widely involved in the regulation of gastric cancer disease progression, which is of some value for disease treatment.