WISP-1与VEGF-A在老年食管鳞癌组织中表达的临床意义

李晨露; 黄俊星

doi:10.3971/j.issn.1000-8578.2024.24.0348

WISP-1与VEGF-A在老年食管鳞癌组织中表达的临床意义

Clinical Significance of WISP-1 and VEGF-A Expression Levels in Elderly Patients with Esophageal Squamous Cell Carcinoma

摘要

摘要:
目的探索WNT1诱导信号通路蛋白-1（WISP-1）及血管内皮生长因子-A（VEGF-A）在老年食管鳞状细胞癌（ESCC）患者中作为新的分子标志物的临床价值。
方法将老年ESCC分为65~70岁组与>70岁组，分别应用免疫组织化学染色法与RT-PCR检测WISP-1、VEGF-A蛋白与mRNA的表达。Log rank检验分析临床病理特征与食管鳞癌预后的关系。
结果 ESCC组织中WISP-1阳性表达率明显高于癌旁组织（53.33% vs. 35.42%，P=0.045）、>70岁及有淋巴结转移的患者显著高于65~70岁（P=0.047）及无淋巴结转移的患者（P=0.032）。ESCC组织中VEGF-A蛋白的阳性表达率显著高于癌旁组织（50.00% vs. 20.83%，P=0.001），浸润程度T3~T4期、有淋巴结转移、TNM分期Ⅲ~Ⅳ期显著高于T1~T2、无淋巴结转移、Ⅰ~Ⅱ期患者（P=0.030、0.006、0.010）。WISP-1 mRNA和VEGF-A mRNA表达水平明显高于癌旁组织（P<0.05）。单因素分析结果表明WISP-1及VEGF-A表达水平与老年ESCC的预后相关（P<0.05），多因素分析结果表明淋巴结转移是影响老年ESCC患者预后的独立危险因素。
结论 WISP-1及VEGF-A与老年ESCC患者恶性行为相关，且年龄越大，WISP-1表达越高，有作为老年ESCC新的分子标志物的潜能。

Abstract:
Objective To investigate the clinical value of WNT1 inducible signaling pathway protein 1 (WISP-1) and vascular endothelial growth factor-A (VEGF-A) as novel molecular markers of esophageal squamous cell carcinoma (ESCC) in elderly patients.
Methods Elderly patients with ESCC were divided into 65-70 years old group and >70 years old group. Immunohistochemical staining and RT-PCR were used to detect the expression levels of WISP-1 and VEGF-A proteins and mRNAs, respectively. Log rank test was used to analyze correlation between clinicopathological features and prognosis of ESCC in the elderly.
Results The positive expression rate of WISP-1 in ESCC tissues was significantly higher than that in para-cancer tissues (53.33% vs. 35.42%, P=0.045). The positive expression rate of WISP-1 in patients over 70 years old and with lymph node metastasis was significantly higher than that in patients aged 65-70 years and without lymph node metastasis (P=0.047 and 0.032). The positive expression rate of VEGF-A protein in ESCC tissues was significantly higher than that in para-cancer tissues (50.00% vs. 20.83%, P=0.001). The positive expression rate of VEGF-A in patients with depth of infiltration T3-T4 stage, lymph node metastasis, and TNM Ⅲ-Ⅳ stage was significantly higher than that in patients with T1-T2 stage, no lymph node metastasis, and Ⅰ-Ⅱ stage (P=0.030, 0.006, and 0.010, respectively). The mRNA expression levels of these two molecules were significantly higher in ESCC tissues than in para-cancer tissues (P<0.05), and the results of univariate analysis showed that the expression levels of WISP-1 and VEGF-A were correlated with the prognosis of ESCC in the elderly (P<0.05). The results of multivariate analysis showed that lymph node metastasis was the independent risk factor affecting the prognosis of elderly patients with ESCC.
Conclusion WISP-1 and VEGF-A are associated with malignant behavior in elderly patients with ESCC. The expression of WISP-1 increased with age, and it has potential as a novel molecular marker of ESCC in elderly patients.

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