SV2B过表达对胶质母细胞瘤生物学行为的影响及其机制

陈倩; 徐长玉; 陈祺宁; 段虎斌

doi:10.3971/j.issn.1000-8578.2024.24.0225

SV2B过表达对胶质母细胞瘤生物学行为的影响及其机制

Effect of SV2B Overexpression on Biological Behavior of Glioblastoma and Its Mechanism

摘要

摘要:
目的探讨SV2B过表达对胶质母细胞瘤细胞生长、侵袭及凋亡的影响及其潜在机制。
方法通过慢病毒转染胶质母细胞瘤u87、u251细胞构建SV2B过表达组，另设空白对照组。CCK-8实验、细胞划痕实验、Transwell侵袭和迁移实验分别检测过表达SV2B对u87、u251细胞增殖、迁移和侵袭能力的影响， qRT-PCR和Westen blot检测SV2B蛋白表达水平。
结果与空白对照组相比，过表达SV2B组胶质母细胞瘤细胞增殖、侵袭及迁移能力显著降低（P<0.05）。
结论 SV2B过表达显著抑制胶质母细胞瘤细胞增殖、侵袭和迁移能力。

Abstract:
Objective To analyze the effect of SV2B overexpression on the growth, invasion, and apoptosis of glioblastoma cells, and to explore its potential mechanism.
Methods We transfected glioblastoma u87 and u251 cells with lentivirus as SV2B overexpression group. And blank control group was set up. The effects of SV2B overexpression on the proliferation, migration, and invasion of u87 and u251 cells were detected by CCK-8 assay, cell scratch assay, Transwell invasion, and Transwell migration assay. The expression level of SV2B protein was detected by qRT-PCR and Western blot.
Results Compared with the blank control group, the proliferation, invasion, and migration abilities of u87 and u251 cells in SV2B overexpression group were significantly reduced (P<0.05).
Conclusion SV2B overexpression significantly inhibits the proliferation, invasion, and migration abilities of glioblastoma cells.

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