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环状RNA hsa_circ_0001922对前列腺癌细胞增殖、迁移和侵袭的影响及其潜在分子机制

Effects of Circular RNA hsa_circ_0001922 on Proliferation, Migration and Invasion of Prostate Cancer Cells and Its Potential Molecular Mechanism

  • 摘要:
    目的 探讨环状RNA hsa_circ_0001922对前列腺癌细胞PC-3增殖、迁移和侵袭的影响及其潜在分子机制。
    方法 qRT-PCR、RNA FISH分别检测hsa_circ_0001922在前列腺癌细胞PC-3中的表达水平及定位。靶向抑制hsa_circ_0001922后,使用克隆形成、Transwell实验及划痕实验检测PC-3细胞增殖、迁移和侵袭的能力;qRT-PCR、Western blot检测抑制hsa_circ_0001922后EMT通路相关分子表达水平。
    结果 环状RNA hsa_circ_0001922在前列腺癌细胞PC-3中表达上升(P < 0.01)且存在于细胞质和细胞核中。靶向抑制hsa_circ_0001922后,细胞增殖、迁移和侵袭能力明显减弱(P < 0.05),E-cadherin mRNA表达水平上升(P < 0.05),而Vimentin mRNA水平下降(P < 0.05)。Western blot检测结果与上述一致(均P < 0.05)。
    结论 环状RNA hsa_circ_0001922可能通过EMT途径促进PC-3细胞的增殖、迁移及侵袭。

     

    Abstract:
    Objective To investigate the effect of circular RNA hsa_circ_0001922 on the proliferation, migration and invasion of prostate cancer cell PC-3 and its underlying molecular mechanism.
    Methods qRT-PCR and RNA FISH were used to detect the expression level and localization of hsa_circ_0001922 in PC-3 cells respectively. After hsa_circ_0001922 was targeted inhibited, clone formation, Transwell assay and scratch assay were used to detect the proliferation, migration and invasion abilities of PC-3 cells. qRT-PCR and Western blot were used to detect the expression levels of EMT pathway-related molecules after inhibiting hsa_circ_0001922.
    Results The expression of circular RNA hsa_circ_0001922 was increased in PC-3 cells (P < 0.01) and it existed in the cytoplasm and nucleus. The invasion, migration and invasion abilities were significantly weakened (P < 0.05) after inhibiting hsa_circ_0001922; the expression of E-cadherin mRNA increased (P < 0.05) while the mRNA level of Vimentin decreased (P < 0.05). The results of Western blot were consistent with the above (both P < 0.05).
    Conclusion The circular RNA hsa_circ_0001922 may promote the proliferation, migration and invasion of PC-3 cells through the EMT pathway.

     

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