CircRNA hsa_circ_0044569在肺癌诊断中的价值

侯岩君; 田进海; 王立斌; 何进喜

doi:10.3971/j.issn.1000-8578.2021.21.0488

CircRNA hsa_circ_0044569在肺癌诊断中的价值

CircRNA hsa_circ_0044569 as A Potential Biomarker for Diagnosis of Lung Cancer

摘要

摘要:
目的使用生物芯片技术筛选出在肺癌中具备诊断价值的标志物。
方法通过circRNA表达谱芯片筛选4对在肺癌中差异表达的circRNA，在另外52对肺癌患者的组织标本中，采用qRT-PCR方法验证筛选的差异circRNA hsa_circ_0044569，同时收集患者临床病例信息。用SPSS23.0对所得数据进行独立样本t检验和ROC曲线分析，获得肺癌患者临床资料与circRNA hsa_circ_0044569表达的关系。
结果 hsa_circ_0044569在肺癌组织中的表达水平高于与其配对的癌旁组织。ROC曲线分析结果显示，hsa_circ_0044569诊断肺癌的临界值值为9.62，AUC为0.758，P < 0.05，敏感度0.712，特异性0.712。hsa_circ_0044569表达与肺癌的病理类型之间具有相关性（P < 0.05）。
结论 hsa_circ_0044569在肺癌中高表达，并与肺癌的病理类型具有相关性，提示其在肺癌早期诊断中具备成为潜在生物标志物的可能。

Abstract:
Objective To screen out the biomarkers with diagnostic value in lung cancer by biochip technology.
Methods We screened four pairs of differentially-expressed circRNAs in lung cancer by circRNA expression profiling chip, and then verified the screened differential circRNA hsa_circ_0044569 by qRT-PCR, and collected clinical case information of patients at the same time. The independent sample t test and ROC curve were used to analyze the relation between the clinical data of lung cancer patients and the expression of circRNA hsa_circ_0044569 on clinical samples.
Results The expression level of hsa_circ_0044569 in lung cancer tissues was higher than that of its paired adjacent tissues. The cutoff value of hsa_circ_0044569 for the diagnosis of lung cancer was 9.62, AUC was 0.758, P < 0.05, sensitivity was 0.712, and specificity was 0.712. The expression of hsa_circ_0044569 was significantly related with the pathological type of lung cancer patients (P < 0.05).
Conclusion hsa_circ_0044569 is highly expressed in lung cancer and related to the pathological type, suggesting that it may become a potential biomarker in the early diagnosis of lung cancer.

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