虫草素抑制JAK2/STAT3通路减轻肺癌大鼠放射性免疫功能损伤

刘建波; 李白羽; 柯善保; 张伟

doi:10.3971/j.issn.1000-8578.2021.21.0094

虫草素抑制JAK2/STAT3通路减轻肺癌大鼠放射性免疫功能损伤

Cordycepin Attenuates Immune Function Injury in A Rat Model of Lung Cancer After Radiotherapy by Inhibiting JAK2/STAT3 Pathway

摘要

摘要:
目的探讨虫草素通过JAK2/STAT3信号通路对肺癌大鼠放射性治疗免疫功能损伤的抑制作用。
方法 50只大鼠建立荷瘤模型，另设正常组（10只）。建模成功大鼠随机分为模型组、放疗组、虫草素组、激动剂组和激动剂+虫草素组，每组10只。比较各组大鼠瘤重、肿瘤体积、抑瘤率、IL-6、TNF-α、脾指数、胸腺指数、T淋巴细胞亚群数量、JAK2、p-JAK2、STAT3和p-STAT3蛋白表达水平。
结果与正常组比较，模型组IL-6、TNF-α、CD8+、p-JAK2、p-STAT3升高，脾指数、胸腺指数、CD4+、CD4+/CD8+降低（P < 0.05）；与模型组比较，放疗组瘤重、肿瘤体积、脾指数、胸腺指数、CD4+、CD4+/CD8+降低，IL-6、TNF-α、CD8+、p-JAK2和p-STAT3升高（P < 0.05）；与放疗组比较，虫草素组瘤重、肿瘤体积、IL-6、TNF-α、CD8+、p-JAK2、p-STAT3降低，抑瘤率、脾指数、胸腺指数、CD4+、CD4+/CD8+升高，激动剂组瘤重、肿瘤体积、IL-6、TNF-α、CD8+、p-JAK2、p-STAT3升高，抑瘤率、脾指数、胸腺指数、CD4+、CD4+/CD8+降低（P < 0.05）；与激动剂+虫草素组比较，虫草素组瘤重、肿瘤体积、IL-6、TNF-α、CD8+、p-JAK2、p-STAT3降低，抑瘤率、脾指数、胸腺指数、CD4+、CD4+/CD8+升高，激动剂组瘤重、肿瘤体积、IL-6、TNF-α、CD8+、p-JAK2、p-STAT3升高，抑瘤率、脾指数、胸腺指数、CD4+、CD4+/CD8+降低（P < 0.05）。
结论虫草素可有效抑制肺癌大鼠放射性治疗免疫功能损伤，其机制可能通过抑制JAK2/STAT3信号通路蛋白表达发挥调控作用。

Abstract:
Objective To investigate the inhibitory effect of cordycepin on immune function injury in lung cancer rats after radiation therapy through JAK2/STAT3 signaling pathway.
Methods Fifty rats were used to establish tumor-bearing model and other 10 rats were taken as normal group. After successful modeling, the rats were randomly divided into model group, radiotherapy group, cordycepin group, agonist group and agonist+cordycepin group (10 rats in each group). We compared tumor weight, tumor volume, tumor inhibition rate, IL-6, TNF-α, spleen index and thymus index, the number of T lymphocyte subsets, JAK2, p-JAK2, STAT3 and p-STAT3 protein expression levels among all groups.
Results Compared with normal group, IL-6, TNF-α, CD8+, p-JAK2 and p-STAT3 in model group were increased, while spleen index, thymus index, CD4+ and CD4+/CD8+ were decreased (P < 0.05). Compared with model group, tumor weight, tumor volume, spleen index, thymus index, CD4+ and CD4+/CD8+ were decreased in radiotherapy group, while IL-6, TNF-α, CD8+, p-JAK2 and p-STAT3 were increased (P < 0.05). Compared with radiotherapy group, tumor weight, tumor volume, IL-6, TNF-α, CD8+, p-JAK2 and p-STAT3 were decreased in cordycepin group, while tumor inhibition rate, spleen index thymus index, CD4+ and CD4+/CD8+ were increased; tumor weight, tumor volume, IL-6, TNF-α, CD8+, p-JAK2 and p-STAT3 protein expression were increased in agonist group, while tumor inhibition rate, spleen index, thymus index, CD4+ and CD4+/CD8+ were decreased (P < 0.05). Compared with agonist+cordycepin group, tumor weight, tumor volume, IL-6, TNF-α, CD8+, p-JAK2 and p-STAT3 were decreased in cordycepin group, while tumor inhibition rate, spleen index, thymus index, CD4+ and CD4+/CD8+ were increased; tumor weight, tumor volume, IL-6, TNF-α, CD8+, p-JAK2 and p-STAT3 were increased in agonist group, while tumor inhibition rate, spleen index, thymus index, CD4+ and CD4+/CD8+ were decreased (P < 0.05).
Conclusion Cordycepin can effectively inhibit the immune function injury in lung cancer rats after radiation therapy, and may play a regulatory role by inhibiting the JAK2/STAT3 signal pathway.

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