2012-2019年中国未知HAdV病毒株分型及基因特征分析

马骁; 刘万红

doi:10.3971/j.issn.1000-8578.2021.20.1416

2012-2019年中国未知HAdV病毒株分型及基因特征分析

马骁,
刘万红

Classification and Gene Features of Unverified HAdV in China, 2012-2019

摘要

摘要:
目的分析11株来自中国尚未明确分型的HAdV病毒株完整基因组的基因特征并分型。
方法从GenBank数据库中下载到67条已明确基因型的HAdV基因组序列和7条HAdV参照基因组序列，将这74条参照序列与11株未明确分型的HAdV病毒株基因组序列进行基因结构、基因同源性以及系统进化等分析，从而确定其分型。
结果从系统进化分析结果预测出6株HAdV可能的分型，再由多序列比对分析进行验证。其中两株HAdV通过多序列比对结果发现与HAdV-B参照基因组序列之间的同源性为97.8%，属于B型HAdV；两株HAdV与HAdV-D参照基因组序列之间的同源性为92.8%，属于D型HAdV；还有两株HAdV的多序列比对结果与系统进化分析预测结果不一致，无法推断其分型。剩余5株未能预测的HAdV通过与7条HAdV参照序列进行多序列比对分析，发现与B型HAdV基因组序列的同源性达到了97.9%，推测这5株HAdV基因组可能属于B型HAdV。
结论从基因角度来对11株未知的HAdV基因组进行分型，预测有7株可能属于B型HAdV，有两株可能属于D型HAdV，还有两株无法判断其分型。其中7株B型HAdV可能适合作为溶瘤腺病毒进行骨肉瘤的治疗。

Abstract:
Objective To analyze the type and gene features of 11 unverified HAdVs in China.
Methods We downloaded HAdV genome sequences of 67 strains with clear genotypes and the HAdV reference genome sequences of 7 strains from GenBank database, analyzed them together with eleven unverified HAdV gene sequences and classified the species of each sequence based on the genetic structure, sequence similarity and phylogenetic analysis.
Results Phylogenetic analysis results predicted the classification of six sequences: two of them belonged to HAdV-B and two belonged to HAdV-D (the gene homology were 97.8% and 92.8% in comparison with reference gene respectively), the rest of two sequences were not conclusive. Multiple sequence alignment was used to predict the species of five HAdVs. All of them belonged to species HAdV-B, the sequence similarity compared with reference gene was 97.9%.
Conclusion Genotyping of the unverified HAdV genome from a genetic point of view predicts that seven strains might belong to species HAdV-B, two strains might belong to species HAdV-D and two strains could not be classified. The seven HAdV-B strains might be suitable for the treatment of osteosarcoma as oncolytic adenovirus.

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