Abstract:
Objective To investigate the effect of co-expression plasmid of MBD2 and VSVM on the proliferation of lung cancer cells and its chemotaxis on DC cells in vitro.
Methods MTT assay was used to observe the proliferation inhibition rate of lung cancer LL/2 cells after plasmid transfection in each group. Cell morphological changes were observed by PI fluorescence staining. Cell apoptosis was detected by Annexin V-PE/7-AAD double staining. MBD2 activity was observed by DC chemotaxis experiments.
Results pVSVM group, pVSVM+pMBD2 co-transfection group and pVSVM-MBD2 double expression plasmid transfection group significantly inhibited the proliferation of LL/2 cells, compared with liposome transfection group; and the cell morphology changed obviously. The cell apoptosis rates of blank control group and pMBD2 transfection group were lower, while those in other transfection groups were significantly increased, and the double expression plasmid had stronger apoptosis induction effect. pMBD2 had a good chemotactic effect on DC.
Conclusion The co-expression plasmid of β-defensin-2 and vesicular stomatitis virus matrix protein could significantly inhibit the proliferation and induce the apoptosis of lung cancer cell line LL/2, and has the chemotactic ability of DC cells activity in vitro.
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