唾液中miRNA-21对早期喉鳞状细胞癌的诊断价值

罗彬瑞; 郭天虹; 黄远帅

doi:10.3971/j.issn.1000-8578.2018.17.1137

唾液中miRNA-21对早期喉鳞状细胞癌的诊断价值

Diagnostic Values of Salivary miRNA-21 for Early Laryngeal Squamous Cell Carcinoma

摘要

摘要:
目的初步探讨喉鳞状细胞癌患者唾液中miRNA-21对早期喉鳞状细胞癌（LSCC）的诊断价值。
方法收集20例喉鳞状细胞癌患者和15例喉息肉患者及15例健康对照组的唾液，采用RT-PCR定量检测比较miRNA-21的相对表达情况，监测比较6例LSCC患者手术前和手术后6月的miRNA-21的变化。
结果喉鳞状细胞癌患者的唾液中miRNA-21的表达水平较健康对照组显著上调（P=0.036）。ROC曲线分析miRNA-21对LSCC的诊断价值发现，唾液中miRNA-21在ROC曲线下面积（AUC）为0.792（95%CI: 0.5353~0.885, P=0.036）。手术后6月的miRNA-21相对表达水平较手术前明显下降（P=0.041）。
结论喉鳞状细胞癌患者唾液中的miRNA-21存在高表达，将来可能有望成为早期诊断喉鳞状细胞癌的指标。

Abstract:
Objective To explore the diagnostic value of salivary miR-21 for patients with laryngeal squamous cell carcinoma(LSCC).
Methods We collected saliva samples from 20 LSCC patients, 15 vocal cord polyp patients and 15 healthy controls were collected. Real-time quantitative reverse transcription PCR was applied to compare the relative expression of miR-21. The changes of miRNA-21 in six LSCC patients before and six months after operation was compared through monitoring analysis.
Results The expression of salivary miRNA-21 in LSCC patients was significantly higher than that in healthy control group (P=0.036). The diagnostic value of miRNA-21 for LSCC by ROC curve analysis showed that the area under the curve (AUC) of the miRNA-21 in saliva was 0.792 (95%CI: 0.5353-0.885, P=0.036). The relative expression level of miRNA-21 was significantly decreased in six LSCC patients beforeand six months after operation(P=0.041).
Conclusion miRNA-21 is highly expressed in the saliva of patients with laryngeal squamous cell carcinoma, and it could be used as an indicator of early diagnosis of laryngeal squamous cell carcinoma in future.

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