Abstract:
Objective To investigate the expression of Ras-related proteins in brain 34 (RAB34) regulated by miR-130b in pancreatic cancer.
Methods We collected 22 paired specimens of primary pancreatic cancer and adjacent normal tissues specimens. Immunohistochemistry, Western blot and qRT-PCR were employed to detect the expression level of RAB34. Meanwhile, the expression of miR-130b was detected by qRT-PCR. Cell transfection method was used to discover the regulation of miR-130b on RAB34 gene. The luciferase reporter assay was applied to identify whether RAB34 was directly targeted by miR-130b.
Results Compared with adjacent tissues specimens, miR-130b was significantly downregulated and its target gene RAB34 was upregulated in pancreatic cancer tissues. Overexpression of miR-130b remarkably inhibited the expression of RAB34. Moreover, the dual luciferase assay revealed that RAB34 was directly targeted by miR-130b.
Conclusion RAB34 might have a considerable potential in prognosis identification of pancreatic cancer and be regulated by miR-130b.
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