Abstract: Colorectal cancer(CRC) is one of the most common malignant tumors, and its incidence is increasing year by year. Intestinal tract is the highest organ of microbial colonization in human body, and the imbalance of intestinal flora plays an important role in the development of CRC. Bacterial biofilm(BF) is the matrix informed by the orderly growth of a variety of intestinal bacteria. Biofilm formation has greatly increased the resistance of intestinal bacteria to fungicides such as antibiotics and host immune defenses. The latest view shows that the formation of bacterial biofilms is a crucial intermediate link in the process that intestinal microbiota induces carcinogenesis in colorectal epithelial cells. In this paper, combining with recent research status at home and abroad, we review the bacterial biofilm promoting carcinogenesis of intestinal epithelial cells.