组蛋白去乙酰化酶抑制剂SAHA与紫杉醇联用诱导宫颈癌HeLa细胞凋亡的实验

张蕾; 刘好; 金平; 张可; 王晓梅; 赵滢滢

doi:10.3971/j.issn.1000-8578.2018.17.0562

组蛋白去乙酰化酶抑制剂SAHA与紫杉醇联用诱导宫颈癌HeLa细胞凋亡的实验

Combination of SAHA and Paclitaxel Induce Apoptosis of Human Cervical Cancer HeLa Cells

摘要

摘要:
目的探讨组蛋白乙酰化抑制剂SAHA联合紫杉醇体外对宫颈癌HeLa细胞增殖抑制效果及其机制。
方法分别设置空白对照组SAHA（10 μmol/L）组、紫杉醇（10 nmol/L）组、SAHA（10 μmol/L）+紫杉醇（10 nmol/L）联合组，采用MTT法检测各组HeLa细胞的抑制率，采用流式细胞术检测HeLa细胞凋亡情况和细胞周期。
结果 MTT结果显示经SAHA组、紫杉醇组和联合组分别处理24、48 h后，与SAHA和紫杉醇组比较，联合组能够显著抑制HeLa细胞的增殖，差异有统计学意义，且二者具有叠加作用（Q=0.861, Q=1.25）；HeLa细胞的凋亡实验结果显示联合组的凋亡率分别高于紫杉醇组、SAHA组；HeLa细胞周期实验发现：联合组处理后的HeLa细胞主要处于G₀/G₁期，提示SAHA与紫杉醇联合使用能够抑制HeLa细胞有丝分裂过程中的DNA合成和复制。
结论 SAHA与紫衫醇联用能够抑制宫颈癌HeLa细胞增殖，增强诱导HeLa细胞凋亡的能力，阻滞细胞周期，最终增强抗肿瘤的能力。

Abstract:
Objective To investigate the inhibition effect of paclitaxel combined with SAHA on the proliferation of human cervical cancer HeLa cells in vitro and related mechanism.
Methods Human cervical cancer HeLa cells were treated with paclitaxel (10 nmol/L), SAHA (10 μmol/L), and paclitaxel (10 nmol/L)+SAHA (10 μmol/L) separately for 24h or 48h. The inhibitory rate of tumor cells was determined by MTT assay. Flow cytometry was used to detect HeLa cells apoptosis and cell cycles.
Results MTT assay showed that the proliferation inhibitory rates of the combination group after 24h or 48h treatment were significantly higher than those of paclitaxel and SAHA groups, moreover, paclitaxel and SAHA had overlapping effects (Q=0.861, Q=1.25). SAHA combined with paclitaxel could significantly increase the apoptotic rate of HeLa cells than SAHA or paclitaxel alone. HeLa cells were randomly in G₀/G₁ phase after SAHA and paclitaxel treatment for 24h, which suggested that SAHA combined with paclitaxel could significantly inhibit DNA synthesis and replication during HeLa cell mitosis.
Conclusion The combination of SAHA and paclitaxel could significantly induce cervical cancer HeLa cells apoptosis, inhibit cell proliferation, block cell cycle and enhance their anti-tumor ability.

HTML全文

参考文献(9)

施引文献

资源附件(0)