原发性肺腺癌中Napsin A、TTF1及SPA蛋白的表达及其与<i>EGFR</i>基因突变的相关性

金琳芳; 浦勇; 浦柯艳; 刘彦魁; 王晓莉; 齐晓薇

doi:10.3971/j.issn.1000-8578.2017.17.0271

原发性肺腺癌中Napsin A、TTF1及SPA蛋白的表达及其与EGFR基因突变的相关性

Napsin A, TTF1 and SPA Protein Expressions and Their Correlation with EGFR Gene Mutations in Primary Lung Adenocarcinoma

摘要

摘要:
目的探讨Napsin A、TTF1、SPA在原发性肺腺癌中的表达及与EGFR基因突变的相关性。
方法免疫组织化学法检测306例原发性肺腺癌组织中Napsin A、TTF1、SPA蛋白的表达。ARMS法同时检测这些组织中EGFR基因是否突变。
结果 Napsin A在原发性肺腺癌中表达率为87.25%，TTF1在原发性肺腺癌中表达率为80.72%，SPA在原发性肺腺癌中表达率为60.46%。EGFR基因在原发性肺腺癌中的突变率为40.20%。TTF1、Napsin A、SPA蛋白表达阳性患者EGFR基因突变率均较表达阴性患者高，差异均有统计学意义（P < 0.01）。
结论我们可以通过检测Napsin A、TTF1和SPA是否表达来简单预测原发性肺腺癌中EGFR基因是否会有突变，这对于一些组织不够用于开展分子检测的患者或不能开展分子检测的医院十分实用。

Abstract:
Objective To observe Napsin A, TTF1 and SPA protein expressions and their correlation with EGFR gene mutation in primary lung adenocarcinoma tissues.
Methods Immunohistochemistrical method was used to detect Napsin A, TTF1 and SPA protein expressions in 306 cases of primary lung adenocarcinoma tissues, while ARMS method was used to detect whether there was EGFR gene mutation in these tissues.
Results Napsin A, TTF1 and SPA expression rates were 87.25%, 80.72% and 60.46% in primary lung adenocarcinoma. EGFR gene mutation rate was 40.20% in primary lung adenocarcinoma. Patients with positive TTF1, Napsin A, SPA expressions had higher EGFR gene mutation rate than those with negative expressions (P < 0.01).
Conclusion We can simply predict whether EGFR gene mutation in primary lung adenocarcinoma will occur by detecting whether Napsin A, TTF1 and SPA protein are expressed, and this is very practical for a patient whose tissues are not enough for molecular detection or a hospital where molecular detection cannot be carried out.

HTML全文

参考文献(15)

施引文献

资源附件(0)