Objective In this study, we constructed a gene-coregulation-network to demonstrate the molecular mechanism of how microRNA regulated the initiation of osteosarcoma. This study may lay a foundation for underlining the molecular mechanism of the occurrence and development of osteosarcoma.

Methods By analyzing the differentially-expressed genes, we collected the profiling data of microRNA gene expression of osteosarcoma and found the target genes of miRNA with significant difference. By utilizing KEGG and GO database, we annotated all the differentially-expressed genes and constructed a gene-coregulation-network.

Results This study screened 52 differentially-expressed microRNA, among which 31 microRNAs were up-regulated and 21 microRNAs were down-regulated; a total of five microRNAs were involved in tumor pathway, and there were a total of 314 target genes. GO annotation and KEGG pathway enrichment analysis results showed that the differentially-expressed genes mainly joined in many cancer-related metabolism pathways. Finally, based on the transcription factor data and Pearson correlation analysis, we constructed a gene-coregulation-network.

Conclusion Based on gene co-regulation analysis, we systemically mine the molecular mechanism of the occurrence and development of osteosarcoma.