微小RNA-340对肝癌细胞增殖和凋亡的影响

邹宁; 袁成良; 刘朝红; 许丹

doi:10.3971/j.issn.1000-8578.2017.17.0063

微小RNA-340对肝癌细胞增殖和凋亡的影响

Effect of MicroRNA-340 on Proliferation and Apoptosis of Hepatocellular Carcinoma Cell Lines

摘要

摘要:
目的探讨过表达miR-340对人肝癌HepG2、MHCC-97L细胞增殖、细胞周期和凋亡的影响。
方法 miR-340 mimic转染后RT-PCR法测定肝癌细胞株miR-340 mRNA水平，Brdu-ELISA试剂盒检测细胞增殖，流式细胞术分析细胞周期，膜联蛋白V-FITC凋亡检测试剂盒分析细胞凋亡。
结果 miR-340在肝癌细胞系中表达降低（P < 0.05）；高表达miR-340后肝癌细胞的存活率受到显著抑制（P < 0.05）；G₀/G₁期细胞增多（P < 0.05）；S期细胞减少（P < 0.05）；高表达miR-340后HepG2和MHCC-97L细胞凋亡受到促进（P < 0.05）。
结论 miR-340在抑制细胞增殖、NF-κB1下调诱导的细胞凋亡中起着重要的作用，提示miR-340表达降低是肝细胞肝癌发生的机制之一。

Abstract:
Objective To explore the effect of miR-340 overexpression on cell proliferation, cell cycle and apoptosis of human hepatocellular carcinoma HepG2 and MHCC-97L cells.
Methods The miR-340 mimic was transiently transfected into HCC cells using Lipofectamine^TM 2000 reagent. The mRNA level of miR-340 in HCC cell lines was determined by RT-PCR. Cell proliferation was detected by Brdu-ELISA kit. Flow cytometry was used to analyze cell cycle. Apoptosis was analyzed by Annexin V-FITC apoptosis detection kit.
Results The expression of miR-340 was decreased in HepG2 and MHCC-97L cells (P < 0.05); miR-340 overexpression significantly inhibited cell survival rate (P < 0.05); the percentage of cells in G₀/G₁ stage was increased and that in the S stage was reduced (both P < 0.05); miR-340 overexpression promoted the apoptosis of HepG2 and MHCC-97L cells also.
Conclusion miR-340 plays an important role in the inhibition of cell proliferation and the apoptosis induced by NF-κB1 down-regulation, suggesting that reduced miR-340 expression is one of the mechanisms of hepatocellular carcinogenesis.

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