细胞周期蛋白G2在人甲状腺乳头状癌K1细胞中的表达及其对K1细胞增殖凋亡的影响

王倩倩; 李伟娟; 刘阁玲; 杨晓琳; 俞芳; 肖红珍; 田烁

doi:10.3971/j.issn.1000-8578.2017.16.1088

细胞周期蛋白G2在人甲状腺乳头状癌K1细胞中的表达及其对K1细胞增殖凋亡的影响

CCNG2 Expression and Its Effect on Proliferation and Apoptosis of Thyroid Papillary Cancer K1 Cells

摘要

摘要:
目的探讨细胞周期蛋白G2（CyclinG2, CCNG2）基因对人甲状腺乳头状癌K1细胞增殖、凋亡的影响。
方法通过瞬时转染技术构建CCNG2基因过量表达的甲状腺癌K1细胞系，应用MTT比色法、流式细胞术分别检测细胞增殖、凋亡情况；Western blot、免疫细胞化学染色法检测CCNG2、P53、MDM2蛋白表达情况。
结果 MTT法及流式细胞术结果表明，CCNG2过表达的甲状腺癌K1细胞增殖抑制及凋亡效应明显（P < 0.05）；Western blot结果显示，在CCNG2基因过表达的甲状腺癌K1细胞中抑癌基因P53的蛋白表达显著上调（P < 0.05），免疫细胞化学染色法检测结果与Western blot检测结果一致。
结论 CCNG2基因具有抑制细胞增殖、促进细胞凋亡的作用，可能是通过CCNG2基因上调P53基因水平实现的。

Abstract:
Objective To examine the effect of CCNG2 on the proliferation and apoptosis of thyroid papillary carcinoma (PTC) K1 cells.
Methods In the case of CCNG2's overexpression in PTC K1 cells, we built cells line by means of transient transfection technology, observed the proliferation and apoptosis of cells by MTT assay and flow cytometry (FCM), and detected the protein expression of CCNG2, P53 and MDM2 by Western blot and immunohistochemistry.
Results MTT assay and flow cytometry (FCM) indicated that CCNG2 overexpression significantly inhibited the proliferation and induced the apoptosis of K1 cells (P < 0.05); Western blot analysis showed that tumor suppressor gene P53 protein expression was significantly raised in K1 cells with CCNG2 gene overexpression(P < 0.05); the immunohistochemistry showed the same result with Western blot analysis.
Conclusion CCNG2 can inhibit the proliferation and promote the apoptosis of PTC K1 cells partly by increasing the protein expression level of P53.

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