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LncRNA KCNQ1OT1在卵巢癌组织中的表达及其临床意义

杜晓琴

杜晓琴. LncRNA KCNQ1OT1在卵巢癌组织中的表达及其临床意义[J]. 肿瘤防治研究, 2017, 44(5): 329-333. DOI: 10.3971/j.issn.1000-8578.2017.05.004
引用本文: 杜晓琴. LncRNA KCNQ1OT1在卵巢癌组织中的表达及其临床意义[J]. 肿瘤防治研究, 2017, 44(5): 329-333. DOI: 10.3971/j.issn.1000-8578.2017.05.004
DU Xiaoqin. Expression and Clinical Significance of LncRNA KCNQ1OT1 in Ovarian Cancer Tissues[J]. Cancer Research on Prevention and Treatment, 2017, 44(5): 329-333. DOI: 10.3971/j.issn.1000-8578.2017.05.004
Citation: DU Xiaoqin. Expression and Clinical Significance of LncRNA KCNQ1OT1 in Ovarian Cancer Tissues[J]. Cancer Research on Prevention and Treatment, 2017, 44(5): 329-333. DOI: 10.3971/j.issn.1000-8578.2017.05.004

LncRNA KCNQ1OT1在卵巢癌组织中的表达及其临床意义

详细信息
    作者简介:

    杜晓琴(1981-),女,硕士,副主任医师,主要从事非编码RNA与妇科肿瘤的研究

  • 中图分类号: R737.31

Expression and Clinical Significance of LncRNA KCNQ1OT1 in Ovarian Cancer Tissues

  • 摘要:
    目的 

    探讨长链非编码RNA KCNQ1重叠转录物1(LncRNA KCNQ1OT1)在卵巢癌组织中的表达及与临床病理特征的关系。

    方法 

    实时荧光定量PCR法检测卵巢癌组织、癌旁组织及细胞中LncRNA KCNQ1OT1表达, 分析其表达与患者临床病理特征和预后的关系。通过转染siRNA下调LncRNA KCNQ1OT1的表达,检测细胞增殖和侵袭能力的变化。

    结果 

    卵巢癌组织及细胞株中LncRNA KCNQ1OT1的表达水平显著高于正常卵巢上皮细胞及正常卵巢组织。高表达LncRNA KCNQ1OT1的患者的总生存期(P < 0.001)和无进展生存时间(P < 0.001)较低表达者缩短。LncRNA KCNQ1OT1的表达与FIGO分期、肿瘤分级和远处转移相关。下调LncRNA KCNQ1OT1的表达能够明显抑制细胞的增殖和侵袭。

    结论 

    LncRNA KCNQ1OT1参与了调节卵巢癌的发生、发展,可能作为卵巢癌患者预后的一个潜在生物标志物和治疗靶点。

     

    Abstract:
    Objective 

    To explore the expression of LncRNA KCNQ1 overlapping transcript 1 (KCNQ1OT1) in ovarian cancer tissues and its relationship with clinicopathological features.

    Methods 

    The expression levels of LncRNA KCNQ1OT1 in ovarian cancer tissues, adjacent normal tissues and ovarian cancer cell lines were assessed by quantitative real-time PCR.Then, the associations of LncRNA KCNQ1OT1 expression levels with clinicopathological features and prognosis were evaluated. In addition, LncRNA KCNQ1OT1 functions in tumor progression and invasion were further determined by siRNA-induced LncRNA KCNQ1OT1 silencing in vitro.

    Results 

    The expression levels of LncRNA KCNQ1OT1 in ovarian cancer tissues and cell lines were significantly higher than those in adjacent non-tumor tissues and normal ovarian epithelial cells. Higher LncRNA KCNQ1OT1 expression levels were associated with a shorter overall survival (P=0.006) and disease-free survival (P=0.001) in ovarian cancer patients. In addition, LncRNA KCNQ1OT1 expression was positively correlated with FIGO stage (P=0.002), tumor grade (P=0.006) and distant metastasis (P < 0.001). Moreover, LncRNA KCNQ1OT1 knockdown in ovarian cancer cells markedly suppressed cell proliferation and invasion.

    Conclusion 

    The LncRNA KCNQ1OT1 is a novel factor involved in ovarian cancer progression, and constitutes a potential prognostic biomarker and therapeutic target for the patients with ovarian cancer.

     

  • 图  1   qRT-PCR法检测LncRNA KCNQ1OT1在卵巢癌细胞 (A) 及卵巢癌组织 (B) 中的表达

    Figure  1   The expression of LncRNA KCNQ10T1 were measured in ovarian cancer cells (A) and ovarian cancer tissues (B) by qRT-PCR

    图  2   LncRNA KCNQ1OT1的表达与卵巢癌患者无进展生存时间及总生存时间的关系

    Figure  2   The relationship of LncRNA KCNQ1OT1 expression with progression free survival and overall survival of ovarian patients

    图  3   下调SKOV3细胞中LncRNA KCNQ1OT1的表达抑制细胞的侵袭和增殖

    Figure  3   Knockdown of LncRNA KCNQ1OT1 inhibited invasion and proliferation of SKOV3 cells

    表  1   LncRNA KCNQ1OT1的表达与卵巢癌患者病理特征的关系

    Table  1   The association of LncRNA KCNQ1OT1 expression with clinical characteristics of ovarian cancer patients

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出版历程
  • 收稿日期:  2016-11-22
  • 修回日期:  2017-02-13
  • 网络出版日期:  2024-01-12
  • 刊出日期:  2017-05-24

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