核转录因子Snail对肝内胆管癌细胞HCCC9810侵袭和迁移能力的影响

康强; 邹浩; 刘立鑫; 王连敏; 朱亚; 石万红; 张小文

doi:10.3971/j.issn.1000-8578.2017.05.001

核转录因子Snail对肝内胆管癌细胞HCCC9810侵袭和迁移能力的影响

Effect of Nuclear Franscription Factor Snail on Invasion and Migration of Intrahepatic Cholangiocarcinoma Cell Line HCCC9810

摘要

摘要:
目的探讨核转录因子Snail对肝内胆管癌细胞HCCC9810侵袭和迁移能力的影响。
方法采用小干扰RNA（si-RNA）技术靶向沉默肝内胆管癌细胞HCCC9810中Snail的表达，通过Transwell和划痕实验检测肝内胆管癌细胞侵袭和迁移能力，采用免疫印迹法检测si-RNA处理前后的胆管癌细胞上皮间质转化相关蛋白E-cadherin和Vimentin表达情况。
结果 Transwell结果显示靶向沉默Snail后HCCC9810细胞的迁移能力下降（P=0.005），侵袭能力减弱（P=0.007），细胞划痕结果表明细胞迁移能力同样降低（P=0.017），免疫印迹结果表明沉默Snail后E-cadherin表达上调（P=0.004），Vimentin表达下调（P=0.001）。
结论 Snail可诱导HCCC9810细胞上皮间质转化，增强其侵袭和迁移能力。

Abstract:
Objective To investigate the effect of nuclear transcription factor Snail on the invasion and migration of HCCC9810 cell line.
Methods The intrahepatic cholangiocarcinoma cell line HCCC9810 was selected as the experimental cell. Small interfering RNA (si-RNA) was used to target the silencing Snail. The invasion and migration abilities of intrahepatic cholangiocarcinoma cells were detected by Transwell and scratch test. The expression of E-cadherin and Vimentin which were epithelial mesenchymal transition (EMT) associated protein in cholangiocarcinoma cells treated with si-RNA were detected by immunoblotting.
Results The migration and invasion abilities of HCCC9810 cell were decreased after the targeted silencing of Snail (P=0.005; P=0.007). Cell scratch results showed that cell migration was also decreased (P=0.017). Immunoblotting results showed that E-cadherin expression was up-regulated (P=0.004) after silencing Snail, whereas Vimentin expression was down-regulated (P=0.001).
Conclusion Snail could induce epithelial mesenchymal transition in HCCC9810 cells, which can enhance invasion and migration abilities of HCCC9810 cells.

HTML全文

参考文献(26)

施引文献

资源附件(0)