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摘要:目的
探讨KRT81基因3' UTR rs3660多态与肺癌遗传易感性之间的关系。
方法使用TaqMan-MGB荧光探针标记法对KRT81基因rs3660遗传变异进行基因分型。用Logistic回归计算rs3660各基因型影响非小细胞肺癌（NSCLC）和小细胞肺癌（SCLC）发病风险的OR值及95%CI。
结果KRT81基因rs3660G＞C变异影响SCLC发病风险（P=0.048），但与NSCLC发病风险无关（P=0.614）。与rs3660GG基因型携带者相比，至少携带一个C等位基因者患SCLC风险显著降低（OR=0.70, 95%CI: 0.49~0.99）。吸烟分层分析显示，在不吸烟组中，至少携带一个C等位基因的非吸烟者具有较低的SCLC发病风险（OR=0.60, 95%CI: 0.36~0.99, P=0.049）。进一步以累计吸烟量来分析，未发现至少携带一个C等位基因的轻度吸烟者（或重度吸烟者）具有较低的SCLC发病风险（OR=0.61, 95%CI: 0.26~1.43, P=0.254）。
结论KRT81基因rs3660G＞C变异影响SCLC发病风险，但与NSCLC发病风险无关。
Abstract:ObjectiveTo explore the association of rs3660 polymorphism in the 3′UTR of KRT81 with the susceptibility for lung cancer.
MethodsThe genotypes of rs3660 were determined by TaqMan-MGB real-time PCR. The OR and 95%CI were estimated by Logistic regression to evaluate the association of rs3660 polymorphism with the risk of non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC).
ResultsKRT81 rs3660G > C variant affected the risk of SCLC (P=0.048), not NSCLC (P=0.614). When compared with the individuals with rs3660GG genotype, a significant decreased risk of developing SCLC was shown in C allele carriers (OR=0.70, 95%CI: 0.49-0.99). When stratified by smoking status, C allele carriers had significantly decreased risk among non-smokers (OR=0.60, 95%CI: 0.36-0.99, P=0.049). We also found that C allele carriers had no significantly decreased risk among light smokers (or heavy smokers) (OR=0.61, 95%CI: 0.26-1.43, P=0.254).
ConclusionKRT81 rs3660G > C polymorphism contributes to the risk of SCLC, not NSCLC.
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Key words:
- KRT81 /
- Genetic variants /
- Lung cancer /
- SNP
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表 1 研究对象基本特征在病例及对照组中的分布情况
Table 1 Distributions of select characteristics in cases and control subjects
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表 2 KRT81 rs3660G > C变异与肺癌发病风险的关系
Table 2 Association of KRT81 rs3660G > C polymorphism with the risk of lung cancer
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表 3 rs3660变异吸烟分层与小细胞肺癌发病风险的关系
Table 3 Risk of KRT81 rs3660 genotypes with SCLC by smoking status
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