Correlation Between Stromal Microenvironment and TLR3 Expression, Prognosis Respectively in Hepatocellular Carcinoma
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摘要:目的
分析肝细胞肝癌（hepatocellular carcinoma, HCC）间质微环境与Toll样受体3（Toll-like receptor 3, TLR3）表达的相关性及其对预后的影响。
方法利用已有专利技术制备人体HCC组织芯片，通过免疫组织化学检测HCC间质中各项指标及HCC细胞中TLR3表达，各因素相关分析和Kaplan-meier生存分析上述指标与TLR3的关系，及其与HCC预后的关系。
结果HCC间质微环境中浸润的T细胞（P=0.002）、Kupffer细胞（P=0.049）、自然杀伤（natural killer, NK）细胞（P=0.000）和树突状细胞（dendritic cells, DCs）（P=0.027）与TLR3表达呈正相关，而肥大细胞（P=0.000）、癌相关肌纤维母细胞（carcinoma-associated fibroblasts, CAFs）（P=0.000）和微血管密度（microvessel density, MVD）（P=0.000）与TLR3表达呈负相关；Kupffer细胞（P=0.013）和NK细胞（P=0.001）与预后呈正相关，而肥大细胞（P=0.008）、CAFs（P=0.000）和MVD（P=0.089）与预后呈负相关。
结论间质微环境在HCC的演进过程中起着重要作用。
Abstract:ObjectiveTo analyse the correlation between the stromal microenvironment and the Toll-like receptor 3(TLR3) expression, the prognosis respectively in hepatocellular carcinoma (HCC).
MethodsThe tissue microarrays of human HCC were prepared with self-owned patent technology. The expression of various indexes in HCC stroma and TLR3 in HCC cells were examined with immunohistochemistry. The correlation between the indexes with TLR3 and theirs relationship with the prognosis of HCC were analyzed by multi-factor correlation analysis as well as survival analysis of Kaplan-Meier.
ResultsT cells, Kupffer cells, natural killer (NK) cells and dendritic cells (DCs) had positive correlation with the TLR3 expression in HCC. Mast cells, carcinoma-associated fibroblasts (CAFs) and microvessel density (MVD) had negative correlation with the TLR3 expression in HCC. Kupffer cells and NK cells were positively related with the prognosis of HCC. Mast cells, CAFs and MVD were negatively related with the prognosis of HCC.
ConclusionThe stromal microenvironment of HCC plays an important role in HCC evolution.
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Key words:
- Hepatocellular carcinoma /
- Microenvironment /
- Toll-like receptor 3(TLR3) /
- Correlation
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图 1 肝细胞肝癌中TLR3的阳性表达 (SP×400)
Figure 1 Positive expression of TLR3 in hepatocellular carcinoma tissues (SP×400)
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图 2 HCC中免疫活性细胞反应 (SP×400)
Figure 2 Immunocompetent cell reaction in HCC tissues (SP×400)
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图 3 HCC中α-SMA阳性表达的癌相关肌纤维母细胞与CD34阳性表达的血管内皮细胞 (SP×400)
Figure 3 Carcinoma-associated fibroblasts (CAFs) with positive α-SMA expression and vascular endothelial cells with positive CD34 expression in HCC (SP×400)
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图 4 HCC伴Kupffer细胞、NK细胞和肥大细胞反应的生存率曲线
Figure 4 Kaplan-Meier survival curves of HCC patients with Kupffer cells, NK cells and Mast cells reaction
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图 5 HCC中CAFs反应的生存率曲线 (P < 0.05)
Figure 5 Kaplan-Meier survival curves of HCC patients with CAFs reaction (P < 0.05)
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图 6 HCC中MVD相关的生存率曲线 (P < 0.05)
Figure 6 Kaplan-meier survival curves of HCC patients with MVD (P < 0.05)
表 1 HCC组织中癌间质免疫活性细胞反应与TLR3表达的相关性
Table 1 Correlation between stromal immunocompetent cell reaction and TLR3 expression in HCC
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表 2 HCC组织中癌间质CAFs反应及微血管密度与TLR3表达的相关性
Table 2 Correlation between stromal CAFs, MVD and TLR3 expression in HCC
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表 3 HCC间质免疫活性细胞反应与预后的相关性
Table 3 Correlation between stromal immunocom petent cell reaction and prognosis in HCC
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表 4 HCC间质CAFs反应及MVD与预后的相关性
Table 4 Correlation between stromal CAFs reaction, MVD and prognosis in HCC
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