Effect of Cisplatin Combined with Buzhong Yiqi Decoction on survivin Expression in Transplantation Tumor of Nude Mice with A549/DDP Cells
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摘要:目的
探讨顺铂联合补中益气汤对A549/DDP荷瘤裸鼠移植瘤survivin蛋白表达的影响。
方法30只BALB/c-nu裸小鼠随机分为5组，A：荷瘤对照组、B：顺铂组、C：顺铂联合低剂量补中益气汤组、D：顺铂联合中剂量补中益气汤组、E：顺铂联合高剂量补中益气汤组。25 d后处死全部裸小鼠。测量移植瘤体积、重量并计算药物抑瘤率；Real-time PCR法检测移植瘤组织survivin mRNA的表达；Western blot法检测移植瘤组织survivin蛋白的表达。
结果顺铂联合低、中、高剂量组均较荷瘤对照组、顺铂组移植瘤体积显著缩小，重量显著减轻，差异有统计学意义；顺铂抑瘤率随着补中益气汤的剂量增加而增大；顺铂组、顺铂联合低剂量组较荷瘤对照组移植瘤组织中survivin的基因和蛋白表达上调，而顺铂联合中、高剂量组较荷瘤对照组survivin的基因和蛋白表达下调，差异均有统计学意义；顺铂联合低、中、高剂量补中益气汤组较顺铂组移植瘤组织中survivin的基因和蛋白表达均下调，差异均有统计学意义。
结论顺铂联合补中益气汤可通过下调survivin的表达改善A549/DDP荷瘤裸鼠移植瘤对顺铂的耐药性。
Abstract:ObjectiveTo explore effect of cisplatin combined with Buzhong Yiqi decoction on survivin expression in transplantation tumor of nude mice with A549/DDP cells.
MethodsThirty BALB/c-nu mice were randomly divided into five groups: A group (tumor control group),B group (cisplatin group),C group (cisplatin with low dose of Buzhong Yiqi decoction),D group (cisplatin with medium dose of Buzhong Yiqi decoction),E group (cisplatin with high dose of Buzhong Yiqi decoction). DDP or DDP and Buzhong Yiqi decoction were treated from 9 to 33 day,lastly the mice were executed. The volume and weight of transplantation tumors were measured; tumor inhibitory rate was calculated. Real-time PCR was used to determine the expression of survivin mRNA; Western blot was adopted to detect the expression of survivin protein.
ResultsThe volume and weight of transplantation tumors in C,D and E groups were significantly smaller than those in A and B groups. Tumor inhibitory rates were increased with the increasing dose of Buzhong Yiqi decoction. Compared with A group,the survivin gene and protein levels of transplantation tumors in B and C groups were higher,however,the results were lower in D and E groups. The survivin gene and protein expressions of transplantation tumors in C,D and E groups were significantly down-regulated,compared with B group.
ConclusionCisplatin combined with Buzhong Yiqi decoction could improve cisplatin resistance of transplantation tumor of nude mice with A549/DDP cells through down-regulating the gene and protein expression of survivin.
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图 1 各组A549/DDP细胞荷瘤裸鼠移植瘤
Figure 1 Transplantation tumor of nude mice with A549/DDP cells in every group
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图 2 各组移植瘤组织中survivin mRNA的表达
Figure 2 survivin mRNA expression of transplantation tumor in each group
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图 3 各组移植瘤组织中survivin蛋白的表达
Figure 3 survivin protein expression of transplantation tumor in each group
表 2 顺铂联合补中益气汤对各组裸小鼠体重的影响 (x±s,n=6)
Table 2 Effect of cisplatin combined with Buzhong Yiqi decoction on body weight of nude mice in each group (x±s,n=6)
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表 3 顺铂联合补中益气汤对各组移植瘤体积、重量及抑瘤率的影响 (x±s,n=6)
Table 3 Effect of cisplatin combined with Buzhong Yiqi decoction on volume,weight and inhibitory rate of transplantation tumor of nude mice in each group (x±s,n=6)
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表 4 顺铂联合补中益气汤对各组移植瘤组织中survivin mRNA和蛋白相对表达量的影响 (x±s,n=6)
Table 4 Effect of cisplatin combined with Buzhong Yiqi decoction on survivin mRNA and protein relative expression of transplantation tumor in each group (x±s,n=6)
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