COX-2、NF-κB、WT-1、PTEN在恶性腹膜间皮瘤中的表达及与预后的关系

梁育飞; 郑国启; 李春英; 宋慧; 邢荣格; 郭忠建

doi:10.3971/j.issn.1000-8578.2016.08.011

COX-2、NF-κB、WT-1、PTEN在恶性腹膜间皮瘤中的表达及与预后的关系

Prognostic Significance of COX-2, NF-κB, WT-1 and PTEN Expression in Malignant Peritoneal Mesothelioma

摘要

摘要:
目的研究恶性腹膜间皮瘤组织中环氧合酶-2（COX-2）、核因子-κB（NF-κB）、Wilm’s瘤基因-1（WT-1）、PTEN的表达及与预后的关系。
方法选取2011—2013年经病理确诊的恶性腹膜间皮瘤33例，应用免疫组织化学SP法检测病变腹膜组织中COX-2、NF-κB、WT-1及PTEN的表达，并分析与年龄、性别、血小板升高、石棉接触、CA125升高、腹水、淋巴结转移、临床分期等指标的关系。对所有患者进行随访，分析标志物与预后的关系。
结果 33例恶性腹膜间皮瘤中，COX-2、NF-κB、WT-1及PTEN的阳性表达率分别为63.64%、51.52%、72.73%和21.21%。4项指标与年龄、性别、血小板升高、石棉接触、CA125升高、腹水、淋巴结转移、临床分期均无相关性（P>0.05）。COX-2和NF-κB分别与PTEN呈负相关。单因素和多因素生存分析显示，PTEN阴性表达患者生存时间短，而COX-2与NF-κB阳性表达患者生存时间短。PTEN是影响恶性腹膜间皮瘤预后的独立风险因素。
结论高表达COX-2及NF-κB提示预后不良，PTEN是影响恶性腹膜间皮瘤的独立预后因素。

Abstract:
Objective To investigate the expression of COX-2, NF-κB, WT-1 and PTEN in malignant peritoneal mesothelioma(MPM) tissues and their relationship with clinical prognosis.
Methods We chose 33 cases of MPM diagnosed by pathology and immunohistochemistry from Jan. 2011 to Dec. 2013. We detected the expression of COX-2, NF-κB, WT-1 and PTEN in peritoneal tissues by immunohistochemistry SP method and analysed the relationship of four markers and age, gender, elevated platelet, asbestos exposure, serum CA125 expression, ascites, lymph node metastasis, clinical stage, etc. All patients were followed up to understand the relationship between the markers and the prognosis.
Results Immunohistochemically, positive COX-2, NF-κB, WT-1 and PTEN expression rates were 63.64%, 51.52%, 72.73% and 21.21% in MPM samples, respectively. However, four markers were not related to age, gender, elevated platelet, asbestos exposure, serum CA125 expression, ascites, lymph node metastasis or clinical stage(P>0.05). The expression of COX-2 and NF-κB were negatively correlated to PTEN respectively. Survival analysis showed the survival time of the patients with negative PTEN expression was short, while the survival time of those with positive COX-2 and NF-κB expression was short. PTEN was the independent prognostic factor of MPM.
Conclusion The patients with COX-2(+)/NF-κB(+) are at higher risk of poor prognosis. PTEN may be useful independent prognostic factor of MPM.

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