Abstract:
Objective To explore the microRNAs which are responsible for the pelvic lymph node metastasis of squamous carcinoma of the cervix (SCC).
Methods The different expression of miRNAs between cancer tissue samples from SCC patients with lymph node metastasis (metastasis group, n=8) and without lymph node metastasis (non-metastasis group, n=16) were analyzed by Affymetrix miRNA 4.0 arrays. Two miRNAs which showed significant differences were validated by real-time quantification PCR.
Results There were five miRNAs whose expression levels were increased in the samples from SCC patients with lymph node metastasis compared with those without lymph node metastasis, in which miR-658 and miR-492 showed significant higher folds of expression level (4.24 and 5.43) than others. The different expression of the above two miRNAs measured by real-time quantificative PCR were consistent with the result of microarrays.
Conclusion The upregulation of miR-658 and miR-492 expression might be related with the progress of pelvic lymph node metastasis from SCC.
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