Effects of Progesterone on Expressions of p57kip2 and Cyclin E in Human Endometrioid Carcinoma Cells JEC
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摘要:目的
探讨孕激素对体外培养的人子宫内膜样癌（endometrioid carcinoma, EC）JEC细胞中p57kip2、Cyclin E表达的影响。
方法分别采用高、中、低三种浓度（1×10-4、1×10-6、1×10-8 mol/L）的孕酮（progesterone, P）处理体外培养的JEC细胞，以未加药JEC细胞作为对照组，作用24、48、72 h后，分别采用光学显微镜和电子显微镜观察JEC细胞的形态变化，MTT法检测JEC细胞的生长情况，Western blot法检测JEC细胞中p57kip2、Cyclin E蛋白的表达。
结果MTT法、光学显微镜、电子显微镜结果显示，中、低浓度P可促进JEC细胞的生长（P < 0.05），高浓度P则抑制JEC细胞的生长（P < 0.05）；Western blot结果显示，P作用于JEC细胞后：（1）作用48、72 h时，随着P浓度的增加，p57kip2蛋白的表达逐渐增高（P > 0.05），Cyclin E蛋白的表达则逐渐降低（P < 0.05）；（2）同一浓度时，随着P作用时间的延长，p57kip2蛋白在高、中、低浓度组中的表达均呈递增趋势（P < 0.05），Cyclin E蛋白在中、高浓度组的表达均呈递减趋势（P < 0.05）；p57kip2和Cyclin E蛋白的表达呈负线性相关关系（P < 0.05）。
结论在EC的发生发展中，孕酮对p57kip2、Cyclin E蛋白的表达均具有一定的调控作用。
Abstract:ObjectiveTo investigate the effects of progesterone on the expressions of p57kip2 and Cyclin E in human endometrioid carcinoma(EC) cells JEC in vitro.
MethodsThe JEC cells cultured in vitro were treated with high, medium and low concentrations(1×10-4, 1×10-6 and 1×10-8mol/L) of progesterone(P). The untreated JEC cells were taken as control group. After 24, 48, 72h, the light microscope and electron microscope were used to observe the morphological changes of JEC cells. MTT was used to test the growth conditions. Western blot was used to test the protein expressions of p57kip2 and Cyclin E in JEC cells.
ResultsMTT, light microscope and electron microscope results showed medium and low concentration of progesterone could promote JEC cells growth (P < 0.05), but high concentration of progesterone could inhibit the growth of JEC cells(P < 0.05). Western blot result showed: (1) when JEC cells were treated with progesterone for 48 and 72h, the protein expression of p57kip2 was increased with the increase of progesterone concentration (P > 0.05) while the protein expression of Cyclin E was decreased (P < 0.05); (2) at the same concentration of progesterone, the protein expression of p57kip2 in the high, medium and low concentration groups were progressively increased with the extension of time (P < 0.05), while the protein expression of Cyclin E were progressively decreased in the high and medium concentration groups (P < 0.05); the positive expression rates of p57kip2 and Cyclin E were negatively linear regression relation (P < 0.05).
ConclusionIn the occurrence and development of EC, progesterone could regulate the protein expressions of p57kip2 and Cyclin E.
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Key words:
- Endometrioid carcinoma(EC) /
- JEC cells /
- Progesterone(P) /
- p57kip2 /
- Cyclin E
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图 1 MTT检测不同浓度P作用后JEC细胞的生长曲线
Figure 1 Growth curves of JEC cells after treated with different concentrations of progesterone detected by MTT
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图 2 P作用72 h时JEC细胞的形态变化(×100)
Figure 2 Morphological changes of JEC cells after treated with progesterone for 72h (×100)
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图 3 JEC细胞P作用72 h的超微结构变化
Figure 3 Ultrastructural changes of JEC cells after treated with progesterone for 72h
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图 4 不同浓度P作用JEC细胞24 h p57kip2和Cyclin E蛋白的表达
Figure 4 Protein expressions of p57kip2 and Cyclin E in JEC cells after treated with different concentrations of progesterone for 24h
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图 5 不同浓度P作用JEC细胞48 h p57kip2和Cyclin E蛋白的表达
Figure 5 Protein expressions of p57kip2 and Cyclin E in JEC cells after treated with different concentrations of progesterone for 48h
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图 6 不同浓度P作用JEC细胞72h p57kip2和Cyclin E蛋白的表达
Figure 6 Protein expressions of p57kip2 and Cyclin E in JEC cells after treated with different concentrations of progesterone for 72h
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