Expressions of MMP-7 mRNA, sMICA, VEGF in Peripheral Blood of Lung Cancer Patients and Their Relationships with Invasion and Metastasis
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摘要:目的
测定肺癌患者外周血中基质金属蛋白酶-7 mRNA（MMP-7 mRNA）、可溶性主要组织相容性复合体Ⅰ类相关分子A （sMICA）、血管内皮生长因子（VEGF）的表达水平，探讨它们与侵袭转移的关系及临床意义。
方法采用荧光定量反转录聚合酶链反应检测110例肺癌患者外周血中MMP-7mRNA表达含量，采用酶联免疫吸附实验检测sMICA、VEGF表达水平，并与40例健康者进行比较。
结果肺癌患者外周血中MMP-7 mRNA、sMICA、VEGF表达水平较正常对照组显著升高（P<0.05）。MMP-7 mRNA高表达含量与患者临床分期相关（P<0.05），VEGF高表达水平与病理分型、组织分化程度相关（P<0.05）。肺癌患者外周血中sMICA与MMP-7 mRNA、VEGF的表达水平呈正相关，同时MMP-7 mRNA与VEGF的表达水平呈正相关（P<0.05），治疗有效（包括手术和化疗）的肺癌患者外周血中MMP-7 mRNA、sMICA、VEGF表达水平显著降低（P<0.05），而复发转移患者的表达水平显著升高（P<0.05）。
结论MMP-7 mRNA、sMICA和VEGF共同参与了肺癌患者肿瘤转移微环境的构成，与肺癌的发生发展、侵袭转移密切相关。
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关键词:
- 基质金属蛋白酶-7 mRNA /
- 可溶性主要组织相容性复合体Ⅰ类相关分子 /
- 血管内皮生长因子 /
- 肺癌 /
- 侵袭 /
- 转移
Abstract:ObjectiveTo test the expression levels of matrix metalloproteinases-7 mRNA(MMP-7), solubleMHC class I-related chain A(sMICA), vascular endothelial growth factor(VEGF) in the peripheral blood oflung cancer patients and determine their relationships with the invasion and metastasis and correspondingclinical significance.
MethodsThe expression of MMP-7 mRNA was determined by reverse transcriptionpolymerasechain reaction (RT-PCR) and the levels of sMICA, VEGF were measured with enzymelinked-immunosorbent assay(ELISA). Totally 110 lung cancer patients were regarded as the experimentalgroup and 40 health persons as the control group.
ResultsCompared with the control group, the levelsof MMP-7 mRNA, sMICA and VEGF in the experimental group were significantly increased (P<0.05).The overexpression of MMP-7 mRNA in lung cancer patients were related to clinical stages P<0.05),the high levels of VEGF were related to histopathological types as well as histological grades (P<0.05).The levels of sMICA had a perfect positive correlation with the levels of MMP-7 mRNA and VEGF,meanwhile, the overexpression of MMP-7 mRNA was favorably correlated to the high levels of VEGF in the peripheral blood of lung cancer patients(P<0.05); the levels of MMP-7 mRNA, sMICA and VEGF weresignificantly decreased by effective treatment including surgery and chemotherapy(P<0.05); the increasedexpressions of MMP-7 mRNA, sMICA and VEGF were found in lung cancer patients with recurrence andmetastasis(P<0.05).
ConclusionMMP-7 mRNA, sMICA and VEGF which participate in the compositionof the tumor metastasis microenvironment might have an association with occurrence, development, invasionand metastasis of lung cancer.
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Key words:
- MMP-7 mRNA /
- sMICA /
- VEGF /
- Lung cancer /
- Invasion /
- Metastasis
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图 1 肺癌患者与正常对照组外周血中MMP-7 mRNA的表达
Figure 1 Expression of MMP-7 mRNA in peripheral blood of lung cancer and healthy control groups
表 1 肺癌患者与正常对照组外周血中MMP-7 mRNA、sMICA、VEGF的表达水平
Table 1 Expression of MMP-7 mRNA,sMICA,VEGF in peripheral blood of lung cancer patients and health controls
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表 2 肺癌患者外周血中MMP-7 mRNA、sMICA、VEGF的表达水平与临床各项指标的关系 (pg/ml)
Table 2 Relationship between expression of MMP-7 mRNA,sMICA,VEGF in peripheral blood and clinical parameters of lung cancer patients (pg/ml)
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表 3 肺癌患者治疗前后外周血中MMP- 7 mRNA、 sMICA、VEGF表达水平的比较
Table 3 Expression of MMP-7 mRNA,sMICA,VEGF in peripheral blood of lung cancer patients before and after treatment
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表 4 初诊时与转移后肺癌患者外周血中MMP-7 mRNA、 sMICA、VEGF表达水平的比较
Table 4 Comparison of expression of MMP-7 mRNA, sMICA,VEGF in peripheral blood of lung cancer patients between early treatment and after metastasis Items Early treatment After metastasis
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