Inhibitory Effect of Thermosensitive Liposomes Combined with Microwave Thermotherapy on Human Cervical Carcinoma Cells HeLa
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摘要:目的
构建阿霉素热敏脂质体（DOX-TSLs），并研究其与微波热疗联合对人宫颈癌HeLa细胞的治疗作用。
方法使用逆向蒸发法制备DOX-TSLs；检测其包封率及体外热敏释放情况；MTT法检测HeLa细胞在化疗、热疗及联合治疗时的存活率；流式细胞术检测联合治疗时的细胞凋亡情况。
结果成功构建DOX-TSLs，平均粒径为（190.7±4.8）nm，包封率88.5%；体外累积释放率实验结果表明，DOX-TSLs可在微波热疗条件下迅速释放药物，30 min药物累积释放率达85%以上；DOX-TSLs可在6 h内完全进入肿瘤细胞内部；DOX-TSLs与微波热疗联合可有效抑制肿瘤细胞增殖；与热疗联合能促进细胞凋亡，联合治疗时细胞凋亡率为（62.2±2.3）%。
结论DOX-TSLs与微波热疗联合治疗人宫颈癌HeLa细胞具有明显协同作用，为宫颈癌的治疗模式提供了新的理论及实验依据。
Abstract:ObjectiveTo construct the thermos-sensitive liposomes loading with doxorubicin drug delivery system (DOX-TSLs) and investigate the effect of its combination with microwave thermotherapy on human cervical carcinoma cells HeLa in vitro.
MethodsReverse evaporation method was used to prepare DOXTSLs; drug loading rate and in vitro release were detected; MTT method was used to detect the inhibition of HeLa cells treated with chemotherapy, thermotherapy and the combination; the apoptosis rate was detected by flow cytometry.
ResultsDOX-TSLs drug delivery system was constructed successfully with the average particles size (190.7±4.8) nm and the efficiency 88.5%; the in vitro release detection revealed that the cumulative release rate could reach 85% with microwave thermotherapy at 30 min; DOX could be delivered into HeLa cells within 6 h by DOX-TSLs; the combination therapy of DOX-TSLs and microwave thermotherapy could inhibit cell proliferation effectively and significantly promote cell apoptosis which could be reach (62.2±2.3) %.
ConclusionDOX-TSLs combined with microwave thermotherapy in the treatment of human cervical carcinoma cells HeLa have a synergistic effect. This therapy provides newly theoretical and experimental basis of human cervical carcinoma.
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Key words:
- TSLs /
- Microwave /
- Thermotherapy /
- Combination therapy
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图 1 DOX紫外全波长扫描图(A)及HPLC图(B)
Figure 1 UV wavelength scanning map(A) and(B) HPLC results of DOX
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图 3 HeLa细胞荧光显微镜摄取结果 (×400)
Figure 3 Fluorescence microscopy results of cellular uptake on HeLa cells (×400)
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