GDF11 is Involved in Human Hepatic Carcinoma Cells SMMC-7721 Proliferation and Sensitivity to DDP
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摘要:目的
研究GDF11（growth differentiation factor 11）过表达对肝细胞癌SMMC-7721细胞体外增殖能力及顺铂敏感度的影响。
方法前期成功构建GDF11过表达的SMMC-7721细胞，同时以空载体感染细胞组及野生型细胞组为对照。CCK-8法检测细胞增殖能力，平板克隆形成实验检测细胞克隆形成能力。CCK-8法检测GDF11过表达对细胞顺铂药物敏感度的影响。
结果Western blot结果验证，已成功构建GDF11过表达的SMMC-7721细胞。GDF11过表达可明显抑制SMMC-7721细胞的体外增殖能力，在96、120及144 h时抑制作用最显著（P<0.05），且实验组细胞较对照组克隆形成能力明显减弱（P<0.05）。GDF11过表达能够增强肝细胞癌SMMC-7721细胞对顺铂的敏感度，顺铂浓度取20 μg/ml及40 μg/ml时，与对照组相比差异有统计学意义（P<0.05）。
结论肝细胞癌SMMC-7721细胞过表达GDF11蛋白后，其体外增殖及克隆形成能力明显降低，对化疗药物顺铂的敏感度增强。
Abstract:ObjectiveTo explore the influence of growth differentiation factor 11 (GDF11) overexpression on hepatic carcinoma cells SMMC-7721 proliferation ability and sensitivity to DDP in vitro.
MethodsPrevious study had successfully built SMMC-7721 cells with GDF11 over-expression. Meanwhile, empty vector infected cells and wild-type cells were treated as controls. Cells proliferation ability was examined by CCK-8 assay, and the proliferation curve was calculated. Colony formation assay was adopted to evaluate colony formation ability. The SMMC-7721 cells were treated with various concentrations of DDP, then the cell viability was measured using CCK-8 assay.
ResultsWestern blot showed SMMC-7721 cells with GDF11 over-expression had successfully built. The overexpression of GDF11 could significantly inhibit the proliferation of SMMC-7721 cells in vitro, and the effect were significant at 96, 120, 144h (P<0.05) . Meanwhile GDF11 over-expression group had lower ability of colony formation than control groups(P<0.05) . The over-expression of GDF11 made SMMC-7721 cells more sensitive to DDP, and the effect were significant at 20 and 40μg/ml DDP (P<0.05) .
ConclusionThe over-expression GDF11 could inhibit the proliferation and colony formation abilities of SMMC-7721 cells, and enhance its sensitivity to DDP.
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Key words:
- Hepatic carcinoma /
- GDF11 /
- Proliferation /
- DDP /
- Sensitivity
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图 1 Western blot检测三种细胞中GDF11蛋白的表达水平
Figure 1 Expression of GDF11 in GDF11-7721, GFP-7721 and WT-7721 cells examined by Western blot
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图 2 CCK-8法检测GDF11蛋白过表达对细胞增殖能力的影响
Figure 2 Effect of GDF11 over-expression on cell proliferation ability examined by CCK-8 assay
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图 3 平板克隆形成实验检测GDF11蛋白过表达对细胞克隆形成能力的影响
Figure 3 Effect of GDF11 over-expression on cells colony formation ability
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