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细胞色素P450 2E1-1239G>C变异对转移性非小细胞肺癌患者预后的影响

杨照环, 曹蕾, 何冰, 裴娜, 吴敬, 张雪梅, 林佳

杨照环, 曹蕾, 何冰, 裴娜, 吴敬, 张雪梅, 林佳. 细胞色素P450 2E1-1239G>C变异对转移性非小细胞肺癌患者预后的影响[J]. 肿瘤防治研究, 2016, 43(2): 133-136. DOI: 10.3971/j.issn.1000-8578.2016.02.008
引用本文: 杨照环, 曹蕾, 何冰, 裴娜, 吴敬, 张雪梅, 林佳. 细胞色素P450 2E1-1239G>C变异对转移性非小细胞肺癌患者预后的影响[J]. 肿瘤防治研究, 2016, 43(2): 133-136. DOI: 10.3971/j.issn.1000-8578.2016.02.008
YANG Zhaohuan, CAO Lei, HE Bing, PEI Na, WU Jing, ZHANG Xuemei, LIN Jia. Association of Cytochrome P450 2E1-1239G>C Genetic Variation with Prognosis of Metastatic Non-small Cell Lung Cancer Patients[J]. Cancer Research on Prevention and Treatment, 2016, 43(2): 133-136. DOI: 10.3971/j.issn.1000-8578.2016.02.008
Citation: YANG Zhaohuan, CAO Lei, HE Bing, PEI Na, WU Jing, ZHANG Xuemei, LIN Jia. Association of Cytochrome P450 2E1-1239G>C Genetic Variation with Prognosis of Metastatic Non-small Cell Lung Cancer Patients[J]. Cancer Research on Prevention and Treatment, 2016, 43(2): 133-136. DOI: 10.3971/j.issn.1000-8578.2016.02.008

细胞色素P450 2E1-1239G>C变异对转移性非小细胞肺癌患者预后的影响

基金项目: 国家自然科学基金(81101483);河北省高等学校创新团队领军人才培育计划(LJRC001);唐山市科技计划项目(14130220a)
详细信息
    作者简介:

    杨照环(1975-),女,本科,主治医师,主要从事肿瘤基础与临床研究

    通讯作者:

    张雪梅,E-mail: jyxuemei@gmail.com

    林佳,E-mail: linjia825@126.com

  • 中图分类号: R734.2

Association of Cytochrome P450 2E1-1239G>C Genetic Variation with Prognosis of Metastatic Non-small Cell Lung Cancer Patients

  • 摘要: 目的 探讨细胞色素P450 CY-1239G>C遗传变异对非小细胞肺癌患者预后的影响。方法 采用聚合酶链反应-限制性片断长度多态性方法(PCR-RFLP)对200例非小细胞肺癌患者的CYP2E1-1239G>C遗传变异进行基因分型。应用Kaplan-Meier法进行单因素生存分析,应用Cox多元回归进行多因素生存分析。结果 在所有非小细胞肺癌患者中,CYP2E1-1239GG基因型患者的中位生存时间为55.7月,高于携带-1239CC或CG基因型患者(24.2月)。无远处转移者中位生存时间为59.0月,显著高于有远处转移患者(23.1月)(P<0.001)。进一步对转移性非小细胞肺癌患者的预后进行分析,发现CYP2E1-1239 CC或CG基因型患者的中位生存时间(13.0月)显著低于GG基因型患者(35.3月)(P=0.005)。Cox回归多因素生存分析显示,CYP2E1-1239G>C变异是转移性非小细胞肺癌预后的独立影响因素(P<0.05)。结论 CYP2E1-1239G>C变异与转移性非小细胞肺癌患者预后密切相关。

     

    Abstract: Objective To investigate the association of genetic variations (-1239G>C) in Cytochrome P450 2E1 gene with the prognosis of metastatic non-small cell lung cancer(NSCLC) patients. Methods We determined the genotypes of CYP2E1-1239G>C variants by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP). Statistical analysis was performed using the Sigma State 11.0 statistical software package. Kaplan-Meier Log-rank analysis and COX regression were used to conduct survival analysis. Results The median survival time of CYP2E1-1239GG genotype carriers was longer than that of carriers with -1239CC or CG genotypes carriers (55.7 vs. 24.2 months). The median survival time of the patients without distant metastasis was significantly longer than that with distant metastasis (59 vs. 23.1 months, P<0.001). We found that the metastatic NSCLC patients with at least one CYP2E1-1239C allele had shorter median survival time compared with those with -1239GG genotype (13 vs. 35.3 months, P=0.005). The Cox model analysis showed that CYP2E1 polymorphism was an independent factor for the prognosis of metastatic NSCLC patients(P<0.05). Conclusion The CYP2E1-1239G>C polymorphism is associated with the prognosis of metastatic NSCLC patients.

     

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出版历程
  • 收稿日期:  2015-03-30
  • 修回日期:  2015-10-25
  • 刊出日期:  2016-02-24

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